# US 5,702,943 A · Process for preparing a biomass on a cereal medium, use of the products so prepared and panification ferment

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 5,702,943 A
- Document ID: US5702943A
- Status: Granted
- Kind: A
- Application number: 08/440,767
- Filed: 1995-05-15
- Granted: 1997-12-30
- Earliest claimed: 1994-05-27
- Assignees: Agrano AG
- Inventors: Aloyse Ehret
- Web page: https://patentgrep.com/patent/US5702943A

## CPC

- C12N 1/185: Saccharomyces isolates
- A21D 8/047: {with yeasts}
- C12N 1/18: Baker's yeast; Brewer's yeast
- C12R 2001/865: Saccharomyces cerevisiae
- Y02E 50/10: Biofuels, e.g. bio-diesel
- Y10S 435/819: Fermentation vessels in series
- Y10S 435/942: Saccharomyces cerevisiae
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A21D: TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS (preservation of flour or dough before baking A23B40/00; preservation of bakery products A23B45/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A biomass which can be used directly as panification ferment without previous separation of the culture medium and the biomass and without addition of industrial yeast is prepared by cultivating at least one strain of yeast in a culture medium specified in the description. Preferably, the said ferment comprises as yeast the strain Saccharomyces cerevisiae steineri DSM 9211.

## Claims

### Claim 1

A process for preparing a biomass on a culture medium, the biomass being directly usable as a panification ferment without separating the biomass from the culture medium and without the addition of industrial yeast, said process comprising:
obtaining the culture medium;
inoculating the culture medium with at least one strain of yeast;
providing an air supply;
cultivating the strain of yeast on the culture medium in the presence of the air supply; and
recovering the medium containing the biomass,
wherein the culture medium comprises a medium free of any chemical additives obtained by;
preparing a dilute aqueous mixture comprising at least whole-flour or wheat germ, the mixture comprising starch and gluten;
adding at least one alpha-amylase and at least one amyloglucosidase to hydrolyze all of the starch in the mixture into fermentable sugar; and
adding at least one proteolytic enzyme of food quality to gently hydrolyze at least part of the gluten in the mixture, thereby obtaining the culture medium.

### Claim 2 (depends on 1)

The process of claim 1, wherein oxygen from the air supply is dissolved in the culture providing an oxygen partial pressure in the culture, the partial pressure of the oxygen dissolved in the culture being controlled by regulating the air supply in accordance with a previously defined slope, maintaining the culture in an aerobic metabolism limited in oxygen and maintaining the alcohol production in the range of 0.5 to 10 grams per liter.

### Claim 3 (depends on 1)

The process of claim 1, wherein oxygen from the air supply is dissolved in the culture providing an oxygen partial pressure in the culture, the partial pressure of the oxygen dissolved in the culture being controlled by regulating the speed of agitation in accordance with a previously defined slope, maintaining the culture in an aerobic metabolism limited in oxygen.

### Claim 4 (depends on 1)

The process of claim 1, wherein the culture is of the discontinued alimented type.

### Claim 5 (depends on 1)

The process of claim 1, wherein the culture is grown without pH regulation.

### Claim 6 (depends on 1)

The process of claim 1, wherein said yeast is a strain of Saccharomyces cerevisiae.

### Claim 7 (depends on 6)

The process of claim 6, wherein said yeast is a strain of Saccharomyces cerevisiae isolated from a natural sponge.

### Claim 8 (depends on 7)

Process of claim 7, wherein said yeast is the strain Saccharomyces cerevisiae steineri DSM 9211.

### Claim 9 (depends on 1)

The process of claim 1, comprising the following steps:
introducing a first culture medium into a bioreactor;
inoculating said first culture medium with the yeast culture previously grown on second culture medium; and
continuously alimenting the bioreactor with the second culture medium;
while maintaining:
a temperature of 30.degree..+-.1.degree. C.;
an aeration controlled by the partial pressure of oxygen; and
an ethanol concentration between 0.5 and 10 grams/liter of culture.

### Claim 10 (depends on 1)

The process of claim 1, wherein obtained product is concentrated by centrifugation or by filtration.