# US 5,702,942 A · Microorganism strains that produce a high proportion of alternan to dextran

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- Number: US 5,702,942 A
- Document ID: US5702942A
- Status: Granted
- Kind: A
- Application number: 08/474,637
- Filed: 1995-06-06
- Granted: 1997-12-30
- Earliest claimed: 1994-08-02
- Assignees: The United States of America as represented by the Secretary of Agriculture
- Inventors: Timothy D. Leathers; G. Thomas Hayman; Gregory L. Cote
- Web page: https://patentgrep.com/patent/US5702942A

## CPC

- C12N 9/1051: {Hexosyltransferases (2.4.1)}
- C12N 1/205: Bacterial isolates
- C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P 19/18: produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
- C12Q 1/04: Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)}
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- G01N 2400/14: alpha-D-Glucans, i.e. having alpha 1,n (n=3,4,6) linkages between saccharide units, e.g. pullulan
- G01N 2400/22: Dextran
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A rapid screening procedure to select microorganism strains that produce a high proportion of alternan to dextran and a high proportion of alternansucrase to dextransucrase is described. Strains selected by the method are useful to produce a high proportion of alternansucrase to dextransucrase and to produce a high proportion of alternan to dextran, either fermentatively by cultivation of the strains on sucrose, or enzymatically by using alternansucrase produced by the strains of the invention. The selected strains can also be used to obtain microorganism strains having further improvements.

## Claims

### Claim 1

A biologically pure culture of a bacterial strain that produces a greater proportion of alternan to dextran, said strain selected from the group-consisting of Leuconostoc mesenteroides NRRL B-21138 and L. mesenteroides NRRL B-21297.

### Claim 2 (depends on 1)

The biologically pure culture of claim 1 wherein said strain is Leuconostoc mesenteroides NRRL B-21138.

### Claim 3 (depends on 1)

The biologically pure culture of claim 1 wherein said strain is Leuconostoc mesenteroides NRRL B-21297.