# US 5,702,940 A · Cellulase producing microorganism ATCC 55702

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- Number: US 5,702,940 A
- Document ID: US5702940A
- Status: Granted
- Kind: A
- Application number: 08/729,817
- Filed: 1996-10-08
- Granted: 1997-12-30
- Earliest claimed: 1995-09-14
- Assignees: Lockheed Martin Energy Systems, Inc.
- Inventors: H. Craig Dees
- Web page: https://patentgrep.com/patent/US5702940A

## CPC

- C12N 1/205: Bacterial isolates
- C11D 3/38645: {containing cellulase}
- C12N 9/2437: {Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)}
- C12N 15/01: Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
- C12R 2001/38: Pseudomonas
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

## Claims

### Claim 1

A bacterial strain having all of the identifying characteristics of ATCC 55702.

### Claim 2 (depends on 1)

The strain of claim 1, wherein the strain is ATCC 55702.

### Claim 3 (depends on 1)

The strain of claim 1, wherein the strain is a mutant of ATCC 55702.

### Claim 4

A bacterial cloning host which is sensitive to ampicillin, tetracycline and UV radiation consisting of ATCC 55702, or a mutant strain thereof, possessing all of the identifying characteristics of said bacterial cloning host.