# US 5,702,939 A · Glucosamine-6-phosphate deaminase and process for producing the same

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- Number: US 5,702,939 A
- Document ID: US5702939A
- Status: Granted
- Kind: A
- Application number: 08/612,491
- Filed: 1996-03-07
- Granted: 1997-12-30
- Earliest claimed: 1995-04-25
- Assignees: Agency of Industrial Science and Technology
- Inventors: Shizu Fujishima; Naoko Yamano
- Web page: https://patentgrep.com/patent/US5702939A

## CPC

- C12N 9/90: Isomerases (5.)
- Y10S 435/909: Vibrio
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A glucosamine 6-phosphate deaminase having specific physicochemical properties. A process for producing the glucosamine 6-phosphate deaminase which comprises incubating a microorganism belonging to the genus Vibrio and harvesting the glucosamine 6-phosphate deaminase from the culture thus obtained.

## Claims

### Claim 1

An isolated and purified glucosamine-6-phosphate deaminase which is produced by a microorganism belonging to the genus Vibrio and identified on the basis of the following physicochemical properties:
(1) function:
acting on the amino group of D-glucosamine 6-phosphate to thereby form D-fructose 6-phosphate;
(2) substrate specificity:
acting on D-glucosamine 6-phosphate but not on D-glucosamine;
(3) optimum pH value:
7.4 to 7.8;
(4) stable pH value:
7.0 to 9.0 (after incubating at 37.degree. C. for 30 minutes, at least 70% of the activity is sustained);
(5) optimum temperature:
37.degree. to 38.degree. C; and
(6) heat stability:
50.degree. C. (after incubating at pH 7.5 for 30 minutes, at least 70% of the activity is sustained).

### Claim 2

A process for producing a glucosamine-6-phosphate deaminase which comprises incubating a microorganism belonging to the genus Vibrio and capable of producing a glucosamine-6-phosphate deaminase and harvesting the glucosamine-6-phosphate deaminase from the culture thus obtained.

### Claim 3 (depends on 2)

A process for producing a glucosamine-6-phosphate deaminase as set forth in claim 2 wherein said microorganism is Vibrio cholerae non-O1.