# US 5,702,938 A · Human tissue plasminogen activator

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- Number: US 5,702,938 A
- Document ID: US5702938A
- Status: Granted
- Kind: A
- Application number: 08/468,974
- Filed: 1995-06-06
- Granted: 1997-12-30
- Earliest claimed: 1982-05-05
- Assignees: Genetech, Inc.
- Inventors: David V. Goeddel; William J. Kohr; Diane Pennica; Gordon A. Vehar
- Web page: https://patentgrep.com/patent/US5702938A

## CPC

- C12N 15/71: Expression systems using regulatory sequences derived from the trp-operon
- C12N 9/003: {Dihydrofolate reductase [DHFR] (1.5.1.3)}
- C12N 9/6459: {t-plasminogen activator (3.4.21.68), i.e. tPA}
- C12Y 304/21069: Protein C activated (3.4.21.69)
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

Human tissue plasminogen activator (t-PA) is produced in useful quantities using recombinant DNA techniques. The invention disclosed thus enables the production of t-PA free of contaminants with which it is ordinarily associated in its native cellular environment. Methods, expression vehicles and various host cells useful in its production are also disclosed.

## Claims

### Claim 1

A DNA molecule which is a cDNA molecule or a recombinant DNA molecule encoding an amino acid sequence comprising the presequence of human tissue plasminogen activator.

### Claim 2 (depends on 1)

A DNA molecule according to claim 1 operably linked to a DNA sequence encoding a tissue plasminogen activator.

### Claim 3 (depends on 1)

A process comprising the step of expressing the DNA according to claim 1.

### Claim 4 (depends on 3)

The process according to claim 3 wherein said expression is conducted in a transfected Chinese hamster ovary cell line.