# US 5,702,935 A · Method and compounds for inhibiting lipid biosynthesis of bacteria and plants

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- Number: US 5,702,935 A
- Document ID: US5702935A
- Status: Granted
- Kind: A
- Application number: 08/234,011
- Filed: 1994-04-28
- Granted: 1997-12-30
- Earliest claimed: 1994-04-28
- Assignees: Albert Einstein College of Medicine of Yeshiva University, a Division of Yeshiva University
- Inventors: James Sacchettini; John Blanchard; William R. Jacobs, Jr.
- Web page: https://patentgrep.com/patent/US5702935A

## CPC

- C12N 9/001: {acting on the CH-CH group of donors (1.3)}
- C30B 7/00: Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions (from molten solvents C30B9/00; by normal or gradient freezing C30B11/00; under a protective fluid C30B27/00)
- C30B 29/58: Macromolecular compounds
- Y10S 435/863: Mycobacterium
- Y10S 435/864: Mycobacterium avium
- Y10S 435/865: Mycobacterium fortuitum
- Y10S 435/866: Mycobacterium smegmatis
- Y10S 435/968: High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C30B: SINGLE-CRYSTAL GROWTH (by using ultra-high pressure, e.g. for the formation of diamonds, B01J3/06); UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL (zone-refining of metals or alloys C22B); PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE (casting of metals, casting of other substances by the same processes or devices B22D; working of plastics B29; modifying the physical structure of metals or alloys C21D, C22F); SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE (for producing semiconductor devices or parts thereof H10); APPARATUS THEREFOR
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Inha enzyme crystals and methods of growing said crystals are presented. Three crystal forms of the Inha enzyme with discrete unit cell parameters were obtained. The crystals of the Inha enzyme are of sufficient size and quality for x-ray crystallographic determination of the three dimensional structure of the Inha enzyme in concert with heavy atom derivatives of said crystals. With the three dimensional structure of the Inha enzyme, compounds which inhibit the biochemical activity of the Inha enzyme may be developed.

## Claims

### Claim 1

A crystallized InhA enzyme in the form of a plate having a space group C2 and unit cell constants of a=101.1 .ANG.; b=83.4 .ANG.; c=192.9 .ANG.; .beta.=95.degree.; and .alpha.=.gamma.=90.degree..

### Claim 2 (depends on 1)

The crystallized InhA enzyme of claim 1, wherein the crystal has a size of about 0.3.times.0.5.times.0.5 mm.

### Claim 3

A crystallized InhA enzyme in the form of a parallelpiped having a space group P2.sub.1 and unit cell constants of a=69.0 .ANG.; b=116.0 .ANG.; c=65.0 .ANG.; .beta.=97.8.degree.; and .alpha.=.gamma.=90.degree..

### Claim 4 (depends on 3)

The crystallized InhA enzyme of claim 3, wherein the crystal has a size of about 0.3.times.0.3.times.0.6 mm.

### Claim 5

A crystallized InhA enzyme in the form of a hexagon having a space group P6.sub.2 22 and unit cell constants of a=b=100.14 .ANG.; c=140.4 .ANG.; .alpha.=.beta.=90.degree.; and .gamma.=120.degree..

### Claim 6 (depends on 5)

The crystallized InhA enzyme of claim 5, wherein the crystal has a size of about 0.5.times.0.5.times.0.2 mm.