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US 5,702,932 AGranted
Microinjection methods to transform arthropods with exogenous DNA
AssigneesUniversity of Florida, University of California
InventorsMarjorie A. Hoy, James K. Presnail
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Abstract
This invention relates to methods for transforming arthropods with exogenous DNA. The methods are useful for arthropods that have not previously been amenable to DNA-mediated transformation, and also provide a simpler and more efficient means of transforming arthropods than have previously been described. The method involves microinjecting a nucleic acid sequence encoding a desired trait into the reproductive tract of a female arthropod before oviposition.
Claims7
A method for producing an arthropod transformed with a heterologous DNA sequence, wherein said method comprises:
- injecting said DNA sequence into the reproductive tract of a gravid female arthropod, wherein said injection is prior to oviposition such that transformed ova result, and wherein said arthropod is member of an arthropod family selected from the group consisting of Aphidiidae, Aphelinidae and Phytoseiidae;
- and
- permitting said transformed ova to develop into a transformed arthropod.
Dependencies are inferred from the claim text.