# US 5,702,919 A · DNA encoding canine granulocyte macrophage colony stimulating factor

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- Number: US 5,702,919 A
- Document ID: US5702919A
- Status: Granted
- Kind: A
- Application number: 08/259,696
- Filed: 1994-06-14
- Granted: 1997-12-30
- Earliest claimed: 1990-11-21
- Assignees: Fred Hutchinson Cancer Research Center
- Inventors: Richard A. Nash; Rainer Storb
- Web page: https://patentgrep.com/patent/US5702919A

## CPC

- C07K 14/535: Granulocyte CSF; Granulocyte-macrophage CSF
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Canine granulocyte macrophage colony stimulating factor (caGM-CSF) corresponding to that found in canine serum and/or tissues, structural variants thereof, genes that encode these materials, related expression vectors and cells, recombinant methods for making caGM-CSF, and veterinary treatments therewith.

## Claims

### Claim 1

An isolated DNA molecule, which encodes Amino Acid Sequence I (SEQ ID NO:1).

### Claim 2

An isolated DNA molecule, which encodes Amino Acid Sequence II (SEQ ID NO:2).

### Claim 3

An isolated DNA molecule, which encodes Amino Acid Sequence III (SEQ ID NO:3).

### Claim 4

An isolated DNA molecule, which comprises the nucleotide sequence I (SEQ ID NO:6).

### Claim 5

An isolated DNA molecule, which comprises the nucleotide sequence II (SEQ ID NO:7).

### Claim 6 (depends on 1, 2, 3, 4, 5)

A recombinant expression vector comprising a DNA molecule according to any one of claims 1-5.

### Claim 7 (depends on 6)

A cell transformed by an expression vector according to claim 6.

### Claim 8 (depends on 7)

A cell according to claim 7, wherein said cell is that of a prokaryote.

### Claim 9 (depends on 7)

A cell according to claim 7, wherein said cell is that of a eukaryote.

### Claim 10 (depends on 9)

A cell according to claim 9, wherein said eukaryote is yeast.

### Claim 11 (depends on 9)

A cell according to claim 9, wherein said eukaryote is a mammal.

### Claim 12 (depends on 11)

A cell according to claim 11, which is a COS cell.

### Claim 13 (depends on 7)

A method of producing a proteinaceous molecule having an activity of canine granulocyte macrophage colony stimulating factor, which comprises culturing a cell according to claim 7 for a time sufficient to produce said proteinaceous molecule.