# US 5,702,907 A · Antibodies to the hepatocellular carcinoma oncogene and immunoassays using the same

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- Number: US 5,702,907 A
- Document ID: US5702907A
- Status: Granted
- Kind: A
- Application number: 08/471,540
- Filed: 1995-06-06
- Granted: 1997-12-30
- Earliest claimed: 1989-12-19
- Assignees: The United States of America as represented by the Department of Health and Human Services
- Inventors: Stringner S. Yang
- Web page: https://patentgrep.com/patent/US5702907A

## CPC

- C07K 16/32: against translation products of oncogenes
- C07K 14/82: Translation products from oncogenes
- C07K 2319/00: Fusion polypeptide
- C07K 2319/40: containing a tag for immunodetection, or an epitope for immunisation
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention relates to an oncoprotein specific for hepatocellular carcinomas and to a nucleotide sequence that codes for such a protein. The invention further relates to screening and diagnostic methodologies (and kits based thereon) that make use of the oncoprotein (or antibodies specific for same) and the nucleotide sequence.

## Claims

### Claim 1

Antibodies specific for a protein having the amino acid sequence as shown in FIG. 1 or a naturally occurring allelic variation of said sequence.

### Claim 2 (depends on 1)

The antibodies according to claim 1, wherein said antibodies are polyclonal.

### Claim 3 (depends on 1)

A diagnostic kit for detecting the presence of a protein in a sample, said protein having the sequence shown in FIG. 1 or naturally occurring allelic variations thereof, comprising a container means having disposed therewithin antibodies according to claim 1.

### Claim 4 (depends on 1)

A method of detecting the presence of a protein in a sample, said protein having the amino acid sequence as shown in FIG. 1 or a naturally occurring allelic variation thereof, comprising:
i) contacting the sample with an antibody according to claim 1 under conditions such that binding of said antibody to said protein can occur and whereby an antibody-protein complex is formed; and
ii) assaying for the presence of said complex.

### Claim 5 (depends on 4)

The method according the claim 4 wherein said antibody is linked to a detectable label.

### Claim 6 (depends on 4)

The method according to claim 4 wherein said sample is a tissue sample.