# US 5,702,897 A · Interaction of proteins involved in a cell death pathway

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- Number: US 5,702,897 A
- Document ID: US5702897A
- Status: Granted
- Kind: A
- Application number: 08/607,269
- Filed: 1996-02-20
- Granted: 1997-12-30
- Earliest claimed: 1994-04-13
- Assignees: The Burnham Institute
- Inventors: John C. Reed; Takaaki Sato
- Web page: https://patentgrep.com/patent/US5702897A

## CPC

- G01N 33/502: {for testing non-proliferative effects}
- C07K 14/4747: {Apoptosis related proteins}
- C12Q 1/6897: involving reporter genes operably linked to promoters
- G01N 33/5008: {for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics}
- G01N 33/68: involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)}
- G01N 2510/00: Detection of programmed cell death, i.e. apoptosis
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention provides methods for detecting an interaction among proteins involved in regulating cell death. The invention also provides a drug screening assay useful for identifying agents that alter an interaction among proteins involved in controlling cell death. The invention further provides a method for identifying novel proteins that are involved in a cell death pathway.

## Claims

### Claim 1

A method of detecting a protein involved in a cell death pathway, comprising the steps of:
a. obtaining a cell that expresses an exogenous protein involved in a cell death pathway, wherein expression of said exogenous protein results in the death of said cell;
b. expressing in said cell a second protein that is suspected of being involved in a cell death pathway; and
c. detecting the survival of said cell, wherein said survival indicates that said second protein is involved in a cell death pathway.

### Claim 2 (depends on 1)

The method of claim 1, wherein said cell is a yeast cell.

### Claim 3 (depends on 1)

The method of claim 1, wherein said exogenous protein is a Bax protein.

### Claim 4 (depends on 1)

The method of claim 1, wherein said second protein is expressed from a recombinant nucleic acid.

### Claim 5 (depends on 1)

The method of claim 1, wherein said second protein is a mutant protein.