# US 5,702,893 A · Hydrophobic nucleic acid probe

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- Number: US 5,702,893 A
- Document ID: US5702893A
- Status: Granted
- Kind: A
- Application number: 08/441,192
- Filed: 1995-05-15
- Granted: 1997-12-30
- Earliest claimed: 1989-06-30
- Assignees: Chiron Corporation
- Inventors: Michael S. Urdea; Thomas Horn
- Web page: https://patentgrep.com/patent/US5702893A

## CPC

- C07H 21/00: Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C12Q 1/6834: Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

A method for binding a target polynucleotide in a sample is provided. The method uses an assay reagent which is a substrate noncovalently bound to a hydrophobic moiety which is part of a polynucleotide capture probe. This assay reagent is contacted with the sample under conditions which permit hybridization between said capture probe and said target polynucleotide, thereby binding the target polynucleotide.

## Claims

### Claim 1

A method for binding a target polynucleotide in a sample to an unsubstituted substrate, said method comprising:
providing an assay reagent comprising a substrate having bound noncovalently thereto a polynucleotide capture probe having a hydrophobic moiety at an end wherein said capture probe is bound to said unsubstituted substrate through said hydrophobic moiety,
contacting said assay reagent with said sample under conditions which permit hybridization between said capture probe and said target polynucleotide, thereby binding the target polynucleotide to the unsubstituted substrate.

### Claim 2 (depends on 1)

The method of claim 1 which further comprises detecting said hybridization.

### Claim 3 (depends on 1)

The method of claim 1 wherein said polynucleotide is purified, said method further comprising the step of separating said hybridized polynucleotide from the rest of said sample.