# US 5,702,728 A · Clam extract preparation, the method of preparation and use thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,702,728 A - Document ID: US5702728A - Status: Granted - Kind: A - Application number: 08/532,663 - Filed: 1995-10-13 - Granted: 1997-12-30 - Earliest claimed: 1993-04-17 - Inventors: Jieliang Bi - Web page: https://patentgrep.com/patent/US5702728A ## CPC - A61K 35/618: Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs - A61P 25/04: Centrally acting analgesics, e.g. opioids - A61P 35/00: Antineoplastic agents - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS ## Abstract The present invention relates to an extract of clam and the preparation method thereof. The clam extract can be used as analgesic agent and/or medicines for the inhibition the growth of tumors, especially for the treatment of cancers. ## Claims ### Claim 1 A composition comprising a clam extract prepared from a clam from the group consisting of Anodonta woodiana Lea, Cristaria plicata, Hyriopsis cumingii or a mixture thereof, which is light yellow, yellow or light brown color, and has characteristic absorption in the range of 250 nm to 278 nm of ultraviolet-visible light absorption spectrum. ### Claim 2 (depends on 1) A process for preparing the clam extract composition of claim 1, comprising the steps of: grinding clam meat; treating the ground clam meat with acid and alcohol; separating the acid-and-alcohol treated clam meat to obtain a clear liquid; and concentrating the clear liquid to give the clam extract composition. ### Claim 3 (depends on 2) The process according to claim 2, further comprising dehydration drying the concentrated substance or the precipitate. ### Claim 4 (depends on 2) The process according to claim 2, wherein pH value is 2.0-5.5 in the acid-and-alcohol treatment. ### Claim 5 (depends on 2) The process according to claim 2, wherein the acid is fatty acid with C.sub.2 -C.sub.8 straight chain or branch chain, or hydrochloric acid. ### Claim 6 (depends on 2) The process according to claim 2, wherein the acid is acetic acid, citric acid, hydrochloric acid or a mixture thereof. ### Claim 7 (depends on 2) The process according to claim 2, wherein the average size of the ground clam meat is less than 1 mm. ### Claim 8 (depends on 2) The process according to claim 2, wherein the alcohol in the acid-and-alcohol treatment step is ethanol. ### Claim 9 (depends on 2) The process according to claim 2, wherein the alkali in the step of alkali treatment of the clear liquid is sodium hydroxide or potassium hydroxide. ### Claim 10 (depends on 9) The process according to claim 9, wherein pH value is 8-13 in the alkali treatment. ### Claim 11 (depends on 9) The process according to claim 9, wherein ethanol can be added in the step of alkali treatment. ### Claim 12 (depends on 3) The process according to claim 3, wherein the separated precipitate after the treatment of clear liquid with alkali is treated with acid before the freeze drying or spray drying. ### Claim 13 (depends on 1) A process for preparing the clam extract composition of claim 1, comprising the steps of: grinding clam meat; treating the ground clam meat with heat in the presence of acid and water; separating the heat-treated ground clam meat to obtain a clear liquid; concentrating the clear liquid to give the clam extract composition. ### Claim 14 (depends on 13) The process according to claim 13, further comprising dehydration drying of the concentrated substances. ### Claim 15 (depends on 13) The process according to claim 13, wherein the temperature of the heat treatment is 48.degree..about.88.degree. C. ### Claim 16 (depends on 2) A clam extract composition prepared according to the process of claim 2. ### Claim 17 (depends on 1) A pharmaceutical composition for treatment of pain, comprising an effective amount of the clam extract composition of claim 1 and one or more pharmalogically acceptable carriers or excipients. ### Claim 18 (depends on 1) A method of treating pair in a patient in need thereof comprising administering an effective amount of the clam extract composition of claim 1. ### Claim 19 (depends on 18) The method of claim 18 wherein said administration comprises local administration of the extract to a part of the patient's body affected by said pain. ### Claim 20 (depends on 1) A process for preparing the clam extract composition of claim 1, comprising the steps of: grinding clam meat; treating the ground clam meat with acid and alcohol; separating the acid-and-alcohol treated clam meat to obtain a clear liquid; and treating the clear liquid with alkali to give, by separation, a precipitate which is the clam extract composition.