# US 5,702,681 A · Method for detecting flukicidal activity

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 5,702,681 A
- Document ID: US5702681A
- Status: Granted
- Kind: A
- Application number: 08/434,610
- Filed: 1995-05-04
- Granted: 1997-12-30
- Earliest claimed: 1989-12-22
- Assignees: American Cyanamid Company
- Inventors: Mary Ehlers Doscher
- Web page: https://patentgrep.com/patent/US5702681A

## CPC

- A61K 49/0004: {Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions}
- A01N 43/36: five-membered rings
- C07D 207/34: with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C07D: HETEROCYCLIC COMPOUNDS (macromolecular compounds C08)

## Abstract

This invention relates to a method for detecting the flukicidal activity of a compound or compounds comprising feeding a portion of liver tissue of an animal treated with the compound or compounds to a free-living flatworm and observing the flatworm for mortality or morbidity.

## Claims

### Claim 1

A method for detecting flukicidal activity of a compound comprising the steps of:
(a) administering a non-lethal dosage of the compound to an animal;
(b) waiting a period of time sufficient to allow the compound to circulate to the animal's liver;
(c) dissecting the animal's liver;
(d) administering a portion of the dissected liver to a medium containing a free-living flatworm wherein the portion is of an amount sufficient for the flatworm to feed upon;
(e) observing the flatworm for feeding upon the dissected liver and;
(f) observing the flatworm for the presence of mortality or morbidity.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the animal is a gerbil.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the free-living flatworms are fresh-water planaria.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the planaria are selected from the group consisting of Dugesia tigrina and Phagocata morgani.

### Claim 5 (depends on 1)

The method according to claim 1, wherein step (a) is conducted by administering the compound in the animal's diet for a period of about four days.

### Claim 6 (depends on 1)

The method according to claim 1, wherein step (a) is conducted parenterally or by oral gavage.

### Claim 7 (depends on 6)

The method according to claim 6, wherein step (c) is conducted from about two hours to about twenty-four hours after step (a).

### Claim 8 (depends on 1)

The method according to claim 1, wherein the medium is sterilized pond water.