# US 5,702,672 A · Apparatus and method for multiple simultaneous synthesis

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- Number: US 5,702,672 A
- Document ID: US5702672A
- Status: Granted
- Kind: A
- Application number: 08/540,512
- Filed: 1995-10-10
- Granted: 1997-12-30
- Earliest claimed: 1992-10-08
- Assignees: Warner-Lambert Company
- Inventors: Sheila H. H. DeWitt; Michael Kell; Michael R. Pavia; John S. Kiely; Mel C. Schroeder; Charles J. Stankovic; Steven Ware
- Web page: https://patentgrep.com/patent/US5702672A

## CPC

- C07K 1/047: {Simultaneous synthesis of different peptide species; Peptide libraries}
- B01J 19/0046: {Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays (synthesis methods per se C40B50/00)}
- C07K 1/045: {using devices to improve synthesis, e.g. reactors, special vessels}
- B01J 2219/00283: Reactor vessels with top opening
- B01J 2219/00308: interchangeably mounted in racks or blocks
- B01J 2219/0031: the racks or blocks being mounted in stacked arrangements
- B01J 2219/00373: Hollow needles
- B01J 2219/00416: Vacuum
- B01J 2219/00423: using filtration, e.g. through porous frits
- B01J 2219/00454: by chemical cleavage from the solid support
- B01J 2219/00493: by sparging or bubbling with gases
- B01J 2219/00495: Means for heating or cooling the reaction vessels
- B01J 2219/005: Beads
- B01J 2219/00585: Parallel processes
- B01J 2219/0059: Sequential processes
- B01J 2219/00596: Solid-phase processes
- B01J 2219/00689: using computers
- B01J 2219/00695: Synthesis control routines, e.g. using computer programs
- B01J 2219/0072: Organic compounds
- B01J 2219/00722: Nucleotides
- B01J 2219/00725: Peptides
- C40B 40/06: Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B 40/10: Libraries containing peptides or polypeptides, or derivatives thereof
- C40B 50/14: Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
- C40B 60/14: for creating libraries
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- C40B: COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES (in silico combinatorial libraries of nucleic acids, proteins or peptides G16B35/00; in silico combinatorial chemistry G16C20/60)

## Abstract

An apparatus for multiple, simultaneous synthesis of compounds which consists of: a reservoir block having a plurality of wells; a plurality of reaction tubes, usually gas dispersion tubes, having filters on their lower ends; a holder block, having a plurality of apertures; and a manifold, which may have ports to allow introduction/maintenance of a controlled environment. The manifold top wall has apertures and a detachable plate with identical apertures. The apparatus is constructed from materials which will accommodate heating, cooling, agitation, or corrosive reagents. Gaskets are placed between the components. Rods or clamps are provided for fastening the components together. Apparatus operation involves placing the filters on the lower ends of the reaction tubes in the reservoir block wells, and the upper ends passing through the holder block apertures and into the manifold.

## Claims

### Claim 1

A reaction tube having an upper end, joint section, an intermediate section and a lower end, said lower end containing a filter device, wherein the reaction tube comprises a gas dispersion tube, the joint section comprises ground glass and the intermediate section comprise a means for equilibration of any pressure differentials within a sealed apparatus.

### Claim 2 (depends on 1)

A gas dispersion tube according to claim 1 in which at least one gas dispersion tube has at least one pressure equalization hole located above the filter on the lower end of the gas dispersion tube.

### Claim 3 (depends on 2)

A gas dispersion tube according to claim 2 in which at least one pressure equalization hole is located from 1 to 250 mm above the filter on the lower end of the gas dispersion tube.

### Claim 4 (depends on 3)

A gas dispersion tube according to claim 3 in which at least one pressure equalization hole has a diameter from 0.3 to 1.0 mm.