# US 5,700,690 A · Compositions and methods for inhibiting fibrogenesis

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- Number: US 5,700,690 A
- Document ID: US5700690A
- Status: Granted
- Kind: A
- Application number: 08/452,259
- Filed: 1995-05-26
- Granted: 1997-12-23
- Earliest claimed: 1995-05-26
- Assignees: The Trustees of the University of Pennsylvania
- Inventors: Eric G. Neilson; Theodore Danoff; Hirokazu Okada; Frank Strutz
- Web page: https://patentgrep.com/patent/US5700690A

## CPC

- C12N 15/113: Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)}
- A61K 38/45: Transferases (2)
- A61K 48/00: Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- C12N 15/1136: {against growth factors, growth regulators, cytokines, lymphokines or hormones}
- C12N 2310/111: spanning the whole gene, or a large part of it
- C12N 2799/022: where the vector is derived from an adenovirus
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

Vectors, or naked DNA, containing a promoter for an FSP1 gene and a downstream gene capable of attenuating fibroblasts and their function are provided. Methods of using these vectors to inhibit tissue injury related to fibrogenesis are also provided.

## Claims

### Claim 1

A vector comprising an FSP1 gene promoter sequence contained in Seq. ID No. 1 operably linked to a DNA sequence of interest, wherein said promoter sequence directs transcription of said DNA sequence of interest in mammalian fibroblasts.

### Claim 2 (depends on 1)

The vector of claim 1, wherein the DNA sequence of interest comprises an antisense cassette.

### Claim 3 (depends on 2)

The vector of claim 2, wherein the antisense cassette encodes an antisense agent targeted to an .alpha.1 or .alpha.2 chain of collagen type I, an .alpha.1 chain of collagen type III, or TGF.beta.1.

### Claim 4 (depends on 1)

The vector of claim 1, wherein the DNA sequence of interest encodes a protein which kills transfected fibroblasts.

### Claim 5 (depends on 1)

The vector of claim 1, wherein the DNA sequence of interest encodes thymidine kinase which kills transfected fibroblasts in the presence of gancyclovir.

### Claim 6 (depends on 1)

The vector of claim 1, wherein the DNA sequence of interest encodes a protein which kills transfected fibroblasts in the presence of gancyclovir or acyclovir.