# US 5,700,684 A · Process for preparing a biomass, use of the biomass so prepared, and panification ferment

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- Number: US 5,700,684 A
- Document ID: US5700684A
- Status: Granted
- Kind: A
- Application number: 08/440,768
- Filed: 1995-05-15
- Granted: 1997-12-23
- Earliest claimed: 1994-05-27
- Assignees: Agrano AG
- Inventors: Aloyse Ehret
- Web page: https://patentgrep.com/patent/US5700684A

## CPC

- C12N 1/20: Bacteria; Culture media therefor
- A21D 8/045: {with a leaven or a composition containing acidifying bacteria}
- A21D 8/047: {with yeasts}
- C12N 1/18: Baker's yeast; Brewer's yeast
- Y02E 50/10: Biofuels, e.g. bio-diesel
- Y10S 435/819: Fermentation vessels in series
- Y10S 435/853: Lactobacillus
- Y10S 435/855: Lactobacillus brevis
- Y10S 435/857: Lactobacillus plantarum
- Y10S 435/942: Saccharomyces cerevisiae
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A21D: TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS (preservation of flour or dough before baking A23B40/00; preservation of bakery products A23B45/00)
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A biomass constituted of yeast and of lactic acid bacteria, which can be used directly as panification ferment without previous separation of the culture medium and the biomass, is prepared by cocultivating at least one strain of yeast and at least one strain of lactic acid bacteria in a mixed and/or sequential culture, in a culture medium specified in the description. Preferably, the said ferment comprises as yeast the strain Saccharomyces cerevisiae steineri DSM 9211 and as lactic acid bacteria one or several strains of Lactobacillus brevis DSM 9209, Lactobacillus plantarum DSM 9208, Leuconostoc mesenteroides DSM 9207 and/or Pediococcus pentosaceus DSM 9210.

## Claims

### Claim 1

A process for preparing a biomass on a culture medium, the biomass being directly useable as a panification ferment without separating the biomass from the culture medium and without the addition of industrial yeast, said process comprising;
obtaining the culture medium;
inoculating the culture medium with at least one strain of yeast and at least one strain of lactic acid bacteria;
providing an air supply;
cultivating the strain of yeast and the strain of lactic acid bacteria on the culture medium in the presence of the air supply; and
recovering the medium containing the biomass;
wherein the culture medium comprises a cereal medium free of any chemical additives obtained by;
preparing a dilute aqueous mixture comprising at least whole-flour or wheat germ, the mixture comprising starch and gluten;
adding at least one alpha-amylase and at least one amyloglucosidase to hydrolyze all of the starch in the mixture into fermentable sugar; and
adding at least one proteolytic enzyme of food quality to gently hydrolyze at least part of the gluten in the mixture, thereby obtaining the culture medium.

### Claim 2 (depends on 1)

The process of claim 1, wherein oxygen from the air supply is diluted in the culture providing an oxygen partial pressure in the culture, the partial pressure of oxygen dissolved in the culture being controlled by regulating the air supply in accordance with a previously defined slope, maintaining the culture in an aerobic metabolism limited in oxygen.

### Claim 3 (depends on 1)

The process of claim 1, wherein oxygen from the air supply is dissolved in the culture, providing an oxygen partial pressure in the culture, the partial pressure of oxygen dissolved in the culture being controlled by regulating the speed of agitation in accordance with a previously defined slope, maintaining the culture in an aerobic metabolism limited in oxygen.

### Claim 4 (depends on 1)

The process of claim 1, wherein said inoculation of the culture medium with at least one strain of yeast and at least one strain of lactic acid bacteria is effected simultaneously.

### Claim 5 (depends on 1)

The process of claim 1, wherein said inoculation of the culture medium with at least one strain of yeast and at least one strain of lactic acid bacteria takes place in a sequential manner.

### Claim 6 (depends on 1)

The process of claim 1, wherein the coculture is of the discontinued alimented type.

### Claim 7 (depends on 1)

The process of claim 1, wherein the culture is grown without pH regulation.

### Claim 8 (depends on 1)

The process of claim 1, wherein said yeast is a strain of Saccharomyces cerevisiae.

### Claim 9 (depends on 1)

The process of claim 1, wherein said yeast is a strain of Saccharomyces cerevisiae isolated from a natural sponge.

### Claim 10 (depends on 9)

The process of claim 9, wherein said yeast is the strain Saccharomyces cerevisiae steineri DSM 9211.

### Claim 11 (depends on 1)

The process of claim 1, wherein said lactic acid bacteria are one or several strains belonging to a genus of the group consisting of Lactobacillus, Leuconostoc or Pediococcus.

### Claim 12 (depends on 11)

The process of claim 11, wherein said lactic acid bacteria are one or several strains belonging to a type of the group consisting of Lactobacillus brevis, Lactobacillus plantarum, Leuconostoc mesenteroides and Pediococcus pentosaceus.

### Claim 13 (depends on 12)

The process of claim 12, wherein said lactic acid bacteria are one or several strains belonging to the group consisting of Lactobacillus brevis DSM 9209, Lactobacillus plantarum DSM 9208, Leuconostoc mesenteroides DSM 9207 and Pediococcus pentosaceus DSM 9210.

### Claim 14 (depends on 1)

The process of claim 1, wherein the coculture is grown in a mixed sequential type of culture.

### Claim 15 (depends on 1)

The process of claim 1, wherein the ethanol content in the culture during growth is held between 0.5 and 10 grams/liter of culture.

### Claim 16 (depends on 1)

The process of claim 1, comprising the following steps:
introducing a first culture medium into a bioreactor;
inoculating said first culture medium with the yeast and the lactic acid bacteria to be cultivated, distributed in a second culture medium;
continuously alimenting the bioreactor with the second culture medium; and
recovering the medium containing the biomass; while maintaining:
a temperature of about 30.degree. C.;
an aeration controlled by the partial pressure of oxygen; and
an ethanol concentration between 0.5 and 10 grams/liter of culture.

### Claim 17 (depends on 1)

The process of claim 1, wherein obtained product is concentrated by centrifugation or by filtration.