# US 5,700,674 A · Mutant uricase, a mutant uricase gene, a novel recombinant DNA, and a process for producing mutant uricase

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- Number: US 5,700,674 A
- Document ID: US5700674A
- Status: Granted
- Kind: A
- Application number: 08/701,952
- Filed: 1996-08-23
- Granted: 1997-12-23
- Earliest claimed: 1995-08-24
- Assignees: Kikkoman Corporation
- Inventors: Yasuji Koyama; Toshio Ichikawa
- Web page: https://patentgrep.com/patent/US5700674A

## CPC

- C12N 9/0046: {with oxygen as acceptor (1.7.3)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to mutant uricase containing the amino acid sequence of wild-type uricase shown in SEQ ID NO: 1 wherein the 165-170th amino acids contain a mutated amino acid sequence, a mutant uricase gene coding for said uricase, a recombinant DNA having said mutant uricase gene integrated into a vector DNA, and a process for producing mutant uricase by culturing a microorganism carrying said recombinant DNA and having the ability to produce mutant uricase in a medium, and then recovering mutant uricase from the culture. The present invention provides stable mutant uricase and the gene coding for said mutant uricase, and further the process of the present invention enables efficient production of stable uricase.

## Claims

### Claim 1

An isolated mutant uricase gene coding for a polypeptide containing the amino acid sequence of wild-type uricase shown in SEQ ID NO: 1, wherein the 165-170th amino acids contain a mutated amino acid sequence.

### Claim 2 (depends on 1)

The mutant uricase gene according to claim 1, wherein said mutated amino acid sequence is shown in SEQ ID NO: 2.

### Claim 3 (depends on 1, 2)

A recombinant DNA having the mutant uricase gene of claim 1 or 2 integrated into a vector DNA.

### Claim 4 (depends on 3)

A process for producing mutant uricase, which comprises culturing a microorganism belonging to the genus Escherichia carrying the recombinant DNA of claim 3 and having the ability to produce mutant uricase in a medium, and then recovering mutant uricase from the culture.