Back to results
US 5,700,669 AGranted
Enzymatic hydrolysis method for the conversion of C-7 sugar to C-7 hydroxyl taxanes
AssigneeBristol-Myers Squibb Company
InventorsRonald L. Hanson, Ramesh N. Patel, Laszlo J. Szarka
Life of this patent
Estimating the term…
Abstract
An enzymatic hydrolysis method, wherein one or more C-7 sugar, preferably C-7 xylosyl-bearing taxanes are contacted with an enzyme or microorganism capable of hydrolyzing said sugar groups to hydroxyl groups.
Claims12
A method for the preparation of at least one taxane of the following formula I containing a hydroxyl group directly bonded at C-7:
- ##STR8## where R.sup.1 is hydroxyl or acyloxy;
- R.sup.2 is acyloxy or hydroxyl;
- and
- R.sup.3 and R.sup.4 are independently alkyl or aryl;
- or a salt thereof,
- comprising the steps of contacting at least one taxane of the following formula II containing a xylosyl group directly bonded at C-7:
- ##STR9## where R.sup.1, R.sup.2, R.sup.3 and R.sup.4 are as defined above, and "xylosyl" is a xylosyl group directly bonded at C-7, or a salt thereof,
- with a microorganism, or an enzyme obtained therefrom, capable of catalyzing the hydrolysis of said C-7 xylosyl group to said C-7 hydroxyl group, effecting said hydrolysis, and obtaining said at least one taxane containing a hydroxyl group directly bonded at C-7, wherein said microorganism belongs to a genus selected from the group consisting of Moraxella, Micrococcus and Bacillus.
5 more elements
Dependencies are inferred from the claim text.