Gene expression regulatory DNA
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsRyoichi Katsumata, Yutaka Takano
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Abstract
The present invention provides a gene expression regulatory DNA and a process for preparing a protein using the same. A DNA derived from the isocitrate lyase (ICL) gene of a coryneform bacterium regulates expression of a structural gene encoding a protein when incorporated into a vector DNA together with said structural gene and introduced into a host coryneform bacterium, and a useful protein can be efficiently produced using the DNA.
Claims1
A process for producing a protein which comprises culturing in a medium a host coryneform bacterium tranformant carrying a recombinant DNA prepared by incorporating into a vector DNA autonomously replicable in said host coryneform bacterium (1) a DNA which is from the isocitrate lyase gene of a coryneform bacterium, said DNA having at least a functional nucleotide sequence of nucleotide sequence 1 to 702 of SEQ ID NO:4, and which regulates expression of a structural gene encoding said protein when incorporated into said vector DNA together with said structural gene and introduced into said host coryneform bacterium and (2) the structural gene encoding the protein;
- allowing the protein to accumulate in the culture;
- and recovering the protein from the culture.
Dependencies are inferred from the claim text.