# US 5,700,462 A · Bacillus cereus strain MS1-9, ATCC 55812

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- Number: US 5,700,462 A
- Document ID: US5700462A
- Status: Granted
- Kind: A
- Application number: 08/747,477
- Filed: 1996-11-12
- Granted: 1997-12-23
- Earliest claimed: 1996-11-12
- Assignees: Wisconsin Alumni Research Foundation
- Inventors: Jo Handelsman; Eric V. Stabb; Lynn M. Jacobson; Robert M. Goodman; David W. Johnson; Kevin P. Smith
- Web page: https://patentgrep.com/patent/US5700462A

## CPC

- C12N 1/205: Bacterial isolates
- A01N 63/22: Bacillus
- C12R 2001/085: Bacillus cereus
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

A novel strain of Bacillus cereus, designated MS1-9, has been isolated from the environment. The strain MS1-9, ATCC 55812, is one of a number of B. cereus strains which are useful as biocontrol agents to combat fungal damping off disease in field crop plants, and strain MS1-9 has exhibited good performance under high disease pressure among a large number of natural isolates in fostering the emergence and growth of alfalfa plants under normal field conditions in the upper midwestern U.S.

## Claims

### Claim 1

A biologically pure culture of a bacteria having the identifying characteristics of Bacillus cereus MS1-9, ATCC 55812.

### Claim 2

An inoculum for application to alfalfa comprising a carrier and an effective quantity of a bacteria having the identifying characteristics of Bacillus cereus MS1-9, ATCC 55812.

### Claim 3

A method for protecting alfalfa in a growing medium from damping off disease comprising the steps of placing in the vicinity of the plant to be protected an effective quantity of a bacteria having the identifying charateristics of Bacillus cereus MS1-9, ATCC 55812.