# US 5,700,370 A · Biological treatment plant controlled by fluorescence sensors

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- Number: US 5,700,370 A
- Document ID: US5700370A
- Status: Granted
- Kind: A
- Application number: 08/532,849
- Filed: 1995-09-22
- Granted: 1997-12-23
- Earliest claimed: 1989-02-28
- Assignees: BioBalance A/S
- Inventors: Kim Helmo
- Web page: https://patentgrep.com/patent/US5700370A

## CPC

- C02F 3/1215: {Combinations of activated sludge treatment with precipitation, flocculation, coagulation and separation of phosphates}
- C02F 3/006: {Regulation methods for biological treatment}
- C02F 3/34: characterised by the microorganisms used
- C12Q 1/04: Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)}
- C02F 2209/02: Temperature
- C02F 2209/06: pH
- C02F 2209/11: Turbidity
- C02F 2209/22: O2
- C02F 2209/36: Biological material, e.g. enzymes or ATP
- C02F 2209/40: Liquid flow rate
- C02F 2209/44: Time
- Y02W 10/10: Biological treatment of water, waste water, or sewage
- Y02W 10/37: using solar energy
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- Y02W: CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT

## Abstract

A waste water purification plant for biological treatment, and optionally mechanical and/or chemical treatment, of biodegradable material present in an aqueous environment is provided. The plant in a biological treatment part contains at least one sensor which measures fluorescent emission and/or variation therein of at least one characteristic biogenic fluorophore present in the biological treatment part which comprises a mixed culture of microorganisms, and which plant further includes a data processing device connected to the at least one sensor and a control device connected to the data processing device. The plant also contains a portion for determining quality and/or quantity of the material to be treated including an additional mixed culture of microorganisms, a sample of the biodegradable material and a further sensor or sensors for measuring fluorescent emission.

## Claims

### Claim 1

A waste water purification plant operable for biological treatment and optionally for mechanical and/or chemical treatment of biodegradable material present in an aqueous environment, which plant in a biological treatment part contains at least one first sensor which is operable for measuring fluorescent emission and/or variations therein of at least one characteristic biogenic fluorophore present in the biological treatment part which comprises a first mixed culture of microorganisms, and which plant further comprises:
a first data processing means connected to the at least one first sensor, which first data processing means is operable for converting a recording fluorescent emission signal to a measurement value and comparing said measurement value to a predetermined set point which indicates optimum or near optimum conditions for the first mixed culture of microorganisms with respect to the biodegradation of the biodegradable material.
a first control means connected to the first data processing means, which first control means is adapted to control the biological and optionally mechanical and/or chemical treatment of the biodegradable material on the basis of the signal obtained from the first data processing means as the result of the comparison performed therein, so as to move subsequent fluorescence measurements toward the pre-determined set point, whereby conditions in the biological treatment part are adapted in the direction of optimum or near optimum conditions for the first mixed culture of microorganisms with respect to biodegradation of the biodegradable material,
said at least one characteristic biogenic fluorophore being one which is present in the first mixed culture of microorganisms and acts as indicator of the metabolic activity of the first mixed culture of microorganisms; and
a portion for determining quality and/or quantity of the biodegradable material which is to be treated in the plant, which portion comprises a biological system comprising a second mixed culture of microorganisms and a sample of the biodegradable material and at least one second sensor which is operable for measuring fluorescent emission and/or variations therein of at least one characteristic biogenic fluorophore present in the biological system.

### Claim 2 (depends on 2)

A waste water purification plant according to claim 2, wherein the at least one characteristic biogenic fluorophore measured by the at least one first sensor each is selected from the group consisting of tryptophan- and tyrosine-containing proteins, tryptophan- and tyrosine-containing peptides, tryptophan- and tyrosine-containing derivatives of amino acids, purines, pyrimidines, nucleosides, nucleotides, nucleic acids, steroids and vitamins and mixtures thereof.

### Claim 3 (depends on 2)

A waste water purification plant according to claim 2, wherein the at least one characteristic biogenic fluorophore measured by the at least one first sensor each is a nicotin-amide adenine dinucleotide selected from the group consisting of NADH and NAPH and mixture thereof.

### Claim 4 (depends on 1)

A waste water purification plant according to claim 1, wherein the portion for determining quality and/or quantity of the biodegradable material which is to be treated in the plant further comprises:
a second data processing means connected to the at least one second sensor, which second data processing means is operable for converting a measured fluorescent emission signal to a measurement value and compares measurement value to a previous measurement value so as to evaluate fluctuations in the quantity and/or quality of the biodegradable material, and optionally,
a second control means connected to the second data processing means, which second control means is capable of adapting a subsequent biological and optionally mechanical and/or chemical treatment of the biodegradable material to the quantity and/or quality of the biodegradable material on the basis of the signal obtained from the second data processing means as the result of the comparison performed therein.

### Claim 5 (depends on 4)

A waste water purification plant according to claim 4, wherein the at least one characteristic biogenic fluorophore measured by the at least one second sensor each is selected from the group consisting of tryptophan- and tyrosine-containing proteins, tryptophan- and tyrosine-containing peptides, tryptophan- and tyrosine-containing derivatives of amino acids, purines, pyrimidines, nucleosides, nucleotides, nucleic acids, steroids and vitamins and mixtures thereof.

### Claim 6 (depends on 5)

A waste water purification plant according to claim 5, wherein the at least one characteristic biogenic fluorophore measured by the at least one second sensor each is a nicotin-amide adenine dinucleotide selected from the group consisting of NADH and NAPH and mixtures thereof.