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AssigneeNovartis Finance Corporation
InventorsJames M. Ligon, Thomas Schupp, James Joseph Beck, Dwight Steven Hill, John Andrew Ryals, Stephen Ting Lam, Philip E. Hammer, Scott Joseph Uknes
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The present invention is directed to the production of an antipathogenic substance (APS) in a host via recombinant expression of the polypeptides needed to biologically synthesize the APS. Genes encoding polypeptides necessary to produce particular antipathogenic substances are provided, along with methods for identifying and isolating genes needed to recombinantly biosynthesize any desired APS. The cloned genes may be transformed and expressed in a desired host organisms to produce the APS according to the invention for a variety of purposes, including protecting the host from a pathogen, developing the host as a biocontrol agent, and producing large, uniform amounts of the APS.
A method for protecting a plant against a phytopathogen, comprising transforming said plant with one or more DNA molecules collectively comprising ORF's 1-4 of a structural gene cluster that encodes all of the biosynthetic enzymes in the biosynthetic pathway of pyrrolnitrin, whereby pyrrolnitrin is produced in said plant in amounts which inhibit said phytopathogen.
A method for protecting a plant against a phytopathogen, comprising transforming said plant with one or more DNA molecules that collectively encode all of the polypetides in the biosynthetic pathway of soraphen, whereby soraphen is produced in said plant in amounts which inhibit said phytopathogen.
Dependencies are inferred from the claim text.