Marek's disease virus nucleotide sequence and methods of use
AssigneeCornell Research Foundation, Inc.
InventorsKarel A. Schat, Kazuhiko Ohashi, Priscilla H. O'Connell
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Abstract
A novel nucleotide sequence in the genome of Marek's disease virus (MDV) containing an open reading frame, whose expression is associated with lytic infection and tumor cell development in MDV-infected cells, is described. Also described is the use of this novel sequence for molecular diagnostics of serotype 1 MDV; and to generate a recombinant MDV by inserting one or more endogenous or exogenous genes, operably linked to a control element for expression, into a region of the MDV genome comprising the novel nucleotide sequence, wherein the insertion interrupts expression of the open reading frame.
Claims17
An isolated and purified nucleic acid molecule encoding a Marek's disease virus polypeptide, wherein the polypeptide consists of the amino acid sequence as shown in SEQ ID NO:
- 1.
An isolated and purified nucleic acid molecule which is a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and an open reading frame located between nucleotide positions 725-1045 of SEQ ID NO:1.
A recombinant serotype 1 Marek's disease virus strain, wherein said strain is genetically engineered by inserting one or more endogenous or exogenous genes into a nucleotide sequence consisting of SEQ ID NO:1 to interrupt expression of L1 ORF located between nucleotide positions 725-1045 of SEQ ID NO:1;
- wherein said one or more genes is operably linked to one or more control elements for expression.
A process of preparing a recombinant Marek's disease virus comprising inserting one or more endogenous or exogenous genes, operably linked to a control element for expression, into a region of a serotype 1 Marek's disease virus genome, wherein said region consists of the nucleotide sequence of SEQ ID NO:1, and wherein said one or more genes and the control element are incorporated into a region of the Marek's disease virus genome, and wherein said incorporation interrupts expression from an L1 ORF present in the genome but allows expression of said one or more incorporated genes.
Dependencies are inferred from the claim text.