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US 5,691,456 AGranted
Reagents and methods for the detection and quantification of thyroxine in fluid samples
AssigneeAbbott Laboratories
InventorsMaciej Adamczyk, Donald D. Johnson, Phillip G. Mattingly, Diana E. Clarisse, Joan D. Tyner, Mary M. Perkowitz
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Abstract
Immunoassay methods and reagents for the specific quantification of thyroxine in a test sample are disclosed employing antibodies prepared with thyroxine derivatives of the formula: ##STR1## wherein P is an immunogenic carrier material and X is a linking moiety. The present invention also describes the synthesis of unique labelled reagent of the formula: ##STR2## wherein Q is a detectable moiety and W is a linking moiety, preferably fluorescein or a fluorescein derivative.
Claims1
A method of making an immunogen of the formula:
- ##STR10## wherein BSA denotes bovine serum albumin, the degree of substitution of BSA by the thyroxine derivative is between 1% to 100%, the thyroxine is an L-isomer, and antibodies directed against the immunogen immunoreact with an L-thyroxine conjugate of the formula:
- ##STR11## where Q is a detectable moiety and W is a linking moiety, the method comprising the steps of:
- (a) N-acetylating L-thyroxine to form .alpha.-N-acetyl L-thyroxine;
- (b) conjugating the .alpha.N-acetyl L-thyroxine to 6-aminocaproic acid to form a L-thyroxine conjugate;
- and
- (c) conjugating the L-thyroxine conjugate of step (b) to bovine serum albumin.
3 more elements
Dependencies are inferred from the claim text.