Reagents and methods for the diagnosis and treatment of diabetes and stiff man syndrome
OwnerSteinunn Baekkeskov, Wiltrud Richter +3inventors
InventorsSteinunn Baekkeskov, Wiltrud Richter, Yuguang Shi, Mark Namchuk, John Kim
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Abstract
The invention provides fragments of GAD.sub.65 that are specifically reactive with at least one class of GAD.sub.65 autoantibody. Most fragments are substantially free of N-terminal amino acids that would otherwise limit solubility. Different fragment contain epitopes for different classes of GAD.sub.65 autoantibodies. The fragments are used in methods of diagnosing and treating insulin dependent diabetes mellitus and stiff man syndrome.
Claims16
A soluble fragment of a GAD65 (glutamic acid decarboxylase) protein that is specifically reactive with a GAD.sub.65 autoantibody, wherein the fragment is at least 99% pure and the autoantibody binds to a conformational epitope of the fragment.
A soluble fragment consisting of amino acids 70 to 101 of a GAD65 protein, wherein the fragment is specifically reactive with a GAD.sub.65 autoantibody diagnostic of stiff man syndrome.
A soluble fragment consisting of amino acids 1 to 20 of a GAD65 protein, wherein the fragment is specifically reactive with a GAD.sub.65 autoantibody diagnostic of stiff man syndrome.
A GAD.sub.65 fusion polypeptide immobilized to a solid support, the fusion polypeptide comprising a GAD.sub.65 polypeptide specifically reactive with a GAD.sub.65 autoantibody, and an extension polypeptide fused to the N-terminal of the GAD.sub.65 polypeptide, wherein the fusion polypeptide is immobilized to the support via a monoclonal antibody to the extension peptide.
A GAD.sub.65 polypeptide immobilized to a solid support, wherein the polypeptide is specifically reactive with a GAD.sub.65 autoantibody, and the polypeptide is immobilized to the support via a monoclonal anti-GAD.sub.65 antibody specifically reactive with an epitope within amino acids 1-244 of the GAD.sub.65 polypeptide.
Dependencies are inferred from the claim text.