Bone implant composition comprising a porous matrix, bone growth promoter proteins, and phosphotyrosyl protein phosphatase inhibitor
OwnerDavid J. Baylink, Rolf Ewers +1inventors
InventorsDavid J. Baylink, Rolf Ewers, Axel Kirsch
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Abstract
A bone growth promoting composition, comprising a content of at least one substance from the group consisting of the growth factors FGF, TGF-.beta., IGF-II, PDGF and biologically active mutants and fragments thereof and bone extracts with the corresponding activity as well as a bone extract with BMP activity. In combination with an appropriate application material, the use of the composition in the preparation of implant material is also described.
Claims4
A method for making a bone implant material comprising:
- providing a porous matrix material;
- and thereafter coating the matrix material with at least one bone growth promoter selected from the group consisting of FGF TGF-.beta., IGF-II, PDGF and BMP, and a phosphotyrosyl protein phosphatase inhibitor selected from the group consisting of vanadate and molybdate salts, with the proviso that when the bone growth promoter is IGF-II, the phosphotyrosyl protein phosphatase inhibitor is not ortho-vanadate.
A method for treating an osseous defect comprising:
- applying a bone implant composition directly to the site of the osseous defect, said bone implant composition comprising:
- (a) a porous matrix material;
- (b) at least one bone growth promoter protein selected from the group consisting of FGF, TGF-.beta., IGF-II, PDGF and BMP;
- and (c) a phosphotyrosyl protein phosphatase inhibitor selected from the group consisting of vanadate and molybdate salts, with the proviso that when the bone growth promoter is IGF-II, the phosphotyrosyl protein phosphatase inhibitor is not ortho-vanadate.
A bone implant composition for application to the site of an osseous defect, said implant composition comprising:
- a porous matrix material selected from the group consisting of hydroxyapatite, algae, coral derivatives and mussel derivatives;
- a bone growth promoting amount of at least one bone growth promoter protein selected from the group consisting of FGF, TGF-.beta., IGF-II, PDGF and BMP;
- and
- an amount of a phosphotyrosyl protein phosphatase inhibitor selected from the group consisting of vanadate and molybdate salts, with the proviso that when the bone growth promoter protein is IFG-II, the phosphotyrosyl protein phosphatase inhibitor is not ortho-vanadate, such that the combination of the bone growth promoter protein and the phosphotyrosyl protein phosphatase inhibitor together provide an improvement in bone growth proliferation as measured by .sup.3 H-thymidine uptake and alkaline phosphatase activity, as compared to a composition containing the porous matrix material and the bone growth promoter protein or the porous matrix material and the phosphotyrosyl protein phosphatase inhibitor alone.
Dependencies are inferred from the claim text.