Improved process for preparing polymyxin B/dextran conjugates
AssigneeNovartis AG
InventorsPrasad Kapa, Gleb Kardash, Andrew Kucerovy, Philip Lake, Paul G. Mattner, Russell C. Petter, Mahavir Prashad, Sushil Sharma
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Abstract
An improved process for producing a water-soluble polymyxin B/dextran conjugate comprises the step of reacting polymyxin B or pharmaceutically acceptable salt thereof with dextran in an aqueous media at a pH of about 9.3 to 10.
Claims23
An improved process for producing a water-soluble conjugate of polymyxin B and dextran comprising the step of reacting polymyxin B or a pharmaceutically acceptable salt thereof with dextran in an aqueous media at a pH of 9.3 to 10.
An improved process for producing a water-soluble conjugate of polymyxin B and dextran comprising:
- a) preparing partially oxidized dextran by reacting dextran with sodium periodate,
- b) reacting polymyxin B or a pharmaceutically acceptable salt thereof with dextran in an aqueous media at a pH of 9.3 to 10 and a temperature of about 30.degree. to 35.degree. C.,
- c) adding sodium borohydride to the resultant aqueous media from step b), and
- d) purifying the resultant polymyxin B-dextran conjugate.
Dependencies are inferred from the claim text.