Fluorometric analysis of chloride ion and chemical sensor therefor
AssigneeCanon Kabushiki Kaisha
InventorsYuji Kawabata, Yoshiyuki Toge
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Abstract
Provided is a fluorometric analytical method for detecting chloride ion in a sample by quenching of fluorescence of a fluorescent dye, the fluorescent dye being one of 3,6-bis(dimethylamino)acridine and a derivative thereof of the formulae ##STR1## wherein R is alkyl having 1 to 30 carbon atoms, and X.sup.- is a halogen ion.
Claims23
A fluorometric analytical method for detecting a chloride ion concentration in a sample liquid comprising the steps of:
- providing a fluorescent dye represented by the following formula (1) or (2) in an aqueous form ##STR5## wherein R is alkyl having 1 to 30 carbon atoms, and X.sup.- is a halogen ion;
- bringing said aqueous fluorescent dye into contact with chloride ions in a sample liquid;
- and
- measuring the amount of fluorescence of said fluorescent dye that is quenched by the chloride ions wherein said method is substantially free from interference from other halide anions.
A chemical sensor for measuring a chloride ion concentration comprising a sensitive membrane in a transparent sample cell, wherein said sensitive membrane contains an immobilized fluorescent dye of the following formula (2) on a surface of the membrane, ##STR6## wherein R is alkyl having 1 to 30 carbon atoms, X.sup.- is a halogen ion and wherein fluorescence of the fluorescent dye is quenched by chloride ion without substantial interference from other halide anions.
A chemical sensor for measuring a chloride ion concentration, comprising a support having a fluorescent dye immobilized on a surface of the support, and a chloride ion concentrating means bonded to the surface of the support, the fluorescent dye being represented by the formula (2) ##STR7## wherein R is alkyl having 1 to 30 carbon atoms, X.sup.- is a halogen ion and wherein fluorescence of the fluorescent dye is quenched by chloride ion without substantial interference from other halide anions.
Dependencies are inferred from the claim text.