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AssigneeKurashiki Boseki Kabushiki Kaisha
InventorsSusumu Katsuen, Kunihiro Ohshima, Ryohei Yamamoto, Toyokazu Nishino
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Animal adhesive cells, particularly human vascular endothelial cells, are cultured in serum-free condition by coating at least one polymer having cell adhesive activity on an inner surface of a culture vessel or surface of a carrier for cell culture, and culturing the cells in the coated vessel or with the coated carrier using a serum-free medium for animal cell culture containing isolated serum albumin, and preferably also transferrin. The polymer is a synthetic polymer modified with a peptide having cell adhesive activity or a natural polymer having cell adhesive activity or a combination thereof. Preferably, the peptide is RGDV, RGDS, RGDN, DGEA or YIGSR and the natural polymer is collagen, gelatin, keratin, fibronectin, vitronectin or laminin. A preferred medium for culturing human vascular endothelial cells is basal medium MCDB 131 or MCDB 107 containing isolated serum albumin, transferrin, hydrocortisone and epithelial growth factor.
A method for culturing human vascular endothelial cells in serum free condition comprising 1) coating at least one polymer having cell adhesive activity to adhere cells to a surface on at least a part of an inner surface of a culture vessel of surface of a carrier for cell culture, wherein said at least one polymer is a synthetic polymer modified with a peptide having cell adhesive activity, or a natural polymer having cell adhesive activity, or a combination thereof, and 2) culturing the human vascular endothelial cells in the coated vessel or with the coated carrier using a serum-free medium for human vascular endothelial cell culture containing at least (i) serum albumin isolated from serum, (ii) transferrin, (iii) hydrocortisone, and (iv) epithelial growth factor whereby initial adhesion of said cells to said polymer coating and viability of said cells are increased.
Dependencies are inferred from the claim text.