Method to produce granulocyte colony stimulating factor from immortalized avian T lymphocytes and method to produce immortalized cells
AssigneesThe United States of America as represented by the Secretary of Agriculture, The Texas A&M University System
InventorsMichael H. Kogut, John R. DeLoach, Larry H. Stanker, Rita B. Moyes, Billy M. Hargis
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Abstract
Immortal avian T lymphocyte cell lines which produce and secrete immune lymphokines are disclosed. These cell lines may be produced from T cells recovered from fowl which have been hyperimmunized in vivo. The activated T cells are first exposed in vitro to a mitogen effective for secondary stimulation thereof, and then virally transformed to produce an immortal cell line. When cultured in vitro, the cell lines produce and secrete immune lymphokines which may be administered to fowl to increase their resistance to infections.
Claims2
An immortal cell line wherein said cell line is selected from the group consisting of ConA-C1 having the ATCC accession number CRL-12135 and ConA-B1 having the ATCC accession number CRL-12357.
Dependencies are inferred from the claim text.