Recombinant nucleic acid containing a response element
AssigneeAmerican Cyanamid Company
InventorsPaul Mak, Sotirios K. Karathanasis
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Abstract
The present invention describes a novel method for screening retinoid X receptor agonists or antagonists, comprising: using a retinoid X receptor expressed by a yeast expression system to screen a compound having a retinoid X receptor agonist or antagonist activity as well as a screen for detecting a compound having retinoid X receptor agonist or antagonist activity, which comprises the steps of (1) providing a yeast strain which expresses the retinoic acid receptor and activates a reporter plasmid containing apolipoprotein AI gene site A or a mutated variant thereof; (2) incubating the compound in suitable media and a colorless chromogenic substrate; and (3) examining the media for development of color.
Claims6
A recombinant nucleic acid comprising the nucleotide sequence ACTGACCCCTTGACCCTG in a forward orientation, identified as SEQ. I.D. NO:9.
A recombinant nucleic acid comprising the nucleotide sequence ACTGACCTTTTGACCCCTG in a forward orientation, identified as SEQ. I.D. NO:11.
A recombinant nucleic acid comprising the nucleotide sequence ACTGAACTTTTGACCCCTG in a forward orientation, identified as SEQ. I.D. NO:8.
Dependencies are inferred from the claim text.