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US 5,691,191 AGranted
Medium for the cultivation of lagenidium giganteum
AssigneeAgraQuest, Inc.
InventorsSherry Darlene Heins, Duane Douglas Ewing, Pamela Gail Marrone
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Abstract
A nutrient medium used in fermentation for increasing the yield of cells or microorganisms is provided. The formulation provided increases the yield of the fungus Lagenidium giganteum two- to three-fold over known media. The medium contains peptone, yeast extract, Proflo.RTM. cottonseed flour, glucose, palm oil, cholesterol, CaCl.sub.2 and MgCl.sub.2.
Claims4
A medium for use in fermentation, consisting essentially of:
- (a) 3.6 g per liter peptone;
- (b) 1.5 to 3 g per liter autolyzed yeast extract;
- (c) 1.6 g per liter Proflo.RTM. cottonseed flour;
- (d) 2.0 to 7.75 g per liter dextrose;
- (e) 2.5 g per liter palm oil;
- (f) 0.2 g per liter cholesterol;
- (g) 0.6 g per liter CaCl.sub.2 .multidot.2H.sub.2 O;
- and
- (h) 0.2 g per liter MgCl.sub.2 .multidot.6H.sub.2 O.
6 more elements
A medium for use in fermentation, consisting essentially of:
- (a) 3.6 g per liter peptone;
- (b) 1.5 to 3 g per liter autolyzed yeast extract;
- (c) 1.6 g per liter Proflo.RTM. cottonseed flour;
- (d) 2.0 to 7.75 g per liter dextrose;
- (e) 2.5 g per liter palm oil;
- (f) 0.2 g per liter cholesterol;
- (g) 0.6 g per liter CaCl.sub.2 .multidot.2H.sub.2 O;
- (h) 0.2 g per liter MgCl.sub.2 .multidot.6H.sub.2 O;
- and
- (i) lecithin up to 2.0 g per liter.
7 more elements
Dependencies are inferred from the claim text.