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AssigneeAmerican Cyanamid Company
InventorsMark Henry Pausch, Bradley A. Ozenberger, John Richard Hadcock, Laura Alicia Price, Eileen Marie Kajkowski, Donald Richard Kirsch, Deborah Tardy Chaleff
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A first aspect of the present invention is directed to a transformed yeast cell containing a first heterologous DNA sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second heterologous DNA sequence which codes for a G protein .alpha. subunit or portions thereof fused to DNA sequences from the yeast G protein a subunit. A second aspect of the present invention is a transformed yeast cell containing a heterologous DNA sequence which codes for a G protein coupled receptor. A third aspect of the present invention is a method of assaying compounds to determine effects on cell growth.
A transformed yeast cell comprising a reporter gene under control of a pheromone-responsive promoter, a heterologous protein-coupled receptor gene, each said gene being under the control of a separate promoter, and a gene mutation causing increased sensitivity to receptor activation selected from the group consisting of:
A transformed yeast cell comprising a reporter gene under control of a pheromone-responsive promoter, a heterologous G protein-coupled receptor gene, each said gene being under the control of a separate promoter, and a mutation at a FAR1 or FUS3 gene that permits transcriptional activation of pheromone-responsive genes without cell cycle arrest.
A transformed yeast cell comprising a reporter gene under control of a pheromone-responsive promoter, a heterologous G protein-coupled receptor gene, each said gene being under the control of a separate promoter, a mutation in a SCG1/GPA1 gene, and a hybrid G.alpha. protein.
A transformed yeast cell having a mutation in a SCG1/GPA1 gene comprising a reporter gene under the control of a pheromone-responsive promoter, and a DNA expression vector capable of expressing a hybrid receptor, said receptor being capable of binding to yeast G protein.
Dependencies are inferred from the claim text.