Lactic acid bacterial suppressor mutants and their use as selective markers and as means of containment in lactic acid bacteria
AssigneeChr. Hansen A/S
InventorsFran.cedilla.oise Dickely, Eric Johansen, Dan Nilsson, Egon Bech Hansen, Per Str.o slashed.man
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Abstract
Useful mutants of lactic acid bacteria or plasmids capable of replicating in lactic acid bacteria, comprising nonsense mutation suppressor-encoding genes, the use of such suppressor genes for confining a replicon to a specific lactic acid bacterium or to a lactic acid bacterium growing in a particular environment and for controlling the number of lactic acid bacterial cells in a particular environment.
Claims26
An isolated pure culture of a lactic acid bacterium comprising a nucleotide sequence comprising a gene encoding a nonsense suppressor, said nucleotide sequence being selected from the group consisting of SEQ ID NO:1, SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:32.
A replicon capable of being replicated in a lactic acid bacterial cell, wherein said replicon comprises a nucleotide sequence comprising a gene encoding a nonsense supressor, and wherein said nucleotide sequence is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:32.
A replicon capable of being replicated in a lactic acid bacterial cell, wherein said replicon comprises a nucleotide sequence comprising a gene encoding a nonsense suppressor, and wherein said replicon comprises SEQ ID NO:36.
A replicon capable of being replicated in a lactic acid bacterial cell, wherein said replicon is pFG1.
Dependencies are inferred from the claim text.