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US 5,691,178 AGranted
Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase
AssigneeNovo Nordisk A/S
InventorsMartin Schulein, Kirsten B.o slashed.egh Levring
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Abstract
A cellulase preparation useful for reducing the harshness of cotton-containing fabrics and for reducing the rate at which such fabrics become harsh comprises about 40% or more (based on the total protein content) of an endoglucanase component with a pH optimum of about 7.5-10.0 with a high CMC-endoase activity and affinity towards cellulose and essentially no cellobiohydrolase activity. The cellulase composition is endogenous to a strain of Humicola, Myceliophthora, or Fusarium.
Claims19
A cellulase preparation which comprises a substantially homogeneous endoglucanase component having
- (a) a pH optimum of about 7.5-10.0;
- (b) a carboxymethyl cellulose (CMC) endoase activity of at least about 10 CMC-endoase units per mg of total protein;
- (c) a cellulose affinity viscosity unit (CAVU) activity of at least about 50% at a pH of 9.0;
- and
- (d) essentially no cellobiohydrolase activity;
- wherein the cellulase preparation is endogenous to a strain of Humicola, Myceliophthora, or Fusarium and wherein the endoglucanase component is present in an mount of at least about 50% by weight of the total cellulase content of the cellulase preparation.
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Dependencies are inferred from the claim text.