Immortalized human fetal osteoblastic cells
AssigneeMayo Foundation for Medical Education and Research
InventorsSteven A. Harris, Thomas C. Spelsberg
Life of this patent
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Abstract
Immortalized normal human fetal osteoblastic cells that express a temperature sensitive mutant of simian virus 40 large T antigen are provided.
Claims15
An immortalized normal human fetal osteoblastic cell which expresses a temperature sensitive mutant of simian virus 40 large T protein antigen.
An immortalized normal human fetal osteoblastic cell having the identifying characteristics of ATCC CRL 11372.
An immortalized normal human fetal osteoblastic cell prepared by a process comprising transfecting a human fetal bone cell with a gene coding for a temperature sensitive mutant of simian virus 40 large T antigen.
An immortalized normal human fetal osteoblastic cell prepared by a process comprising transfecting a human fetal bone cell with expression vector pUCSVtsA58.
A method of preparing an immortalized normal human fetal osteoblastic cell comprising:
- (a) isolating a bone cell from human fetal tissue;
- and
- (b) transfecting the isolated human fetal tissue cell with an expression vector comprising a gene coding for a temperature sensitive mutant of simian virus 40 large T antigen to yield said immortalized normal human fetal osteoblastic cell which expresses said antigen.
Dependencies are inferred from the claim text.