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US 5,691,170 AGranted
Generation of hybrid genes and proteins by virus-mediated recombination
AssigneeTherion Biologics Corporation
InventorsLinda R. Gritz, Dennis L. Panicali
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AssigneeTherion Biologics Corporation
InventorsLinda R. Gritz, Dennis L. Panicali
Estimating the term…
The ability of viruses to undergo recombination within tandemly arranged homologous sequences can be utilized to generate chimeric genes and proteins. Tandemly arranged homologous sequences will rapidly degenerate in a random fashion to yield a single copy comprised of portions of both original sequences. Therefore, a recombinant virus which contains two related but non-identical genes in tandem array yields a population of recombinant viruses which contain a spectrum of hybrid sequences derived from recombination between the original genes. The viruses, therefore, contain hybrid DNA sequences that encode proteins with new epitopes or different combinations of epitopes. Vaccines are derived which may afford protection against a broad spectrum of antigen types.
A method of generating a family of hybrid genes, comprising the steps of:
A family of hybrid genes produced by recombinant techniques from at least two tandemly arranged DNA sequences, wherein the tandemly arranged DNA sequences contain encoding regions, and wherein the sequences are non-identical but share a region of sequence identity in an encoding region of the sequences of at least 10 nucleotides, wherein at least one hybrid gene in the family of hybrid genes has the structure:
Dependencies are inferred from the claim text.