DNA encoding allergenic proteins and peptides from Johnson grass pollen
AssigneesThe University of Melbourne, ImmuLogic Pharmaceutical Corporation
InventorsAsil Avjioglu, Mohan Bir Singh, Robert Bruce Knox
Life of this patent
Estimating the term…
Abstract
The present invention provides a nucleic acid having a nucleotide sequence coding for Sor h I, a major allergen of Sorghum halepense, and fragments thereof. The present invention also provides purified Sor h I or at least one fragment thereof, produced in a host cell transformed with a nucleic acid sequence coding for Sor h I, or at least one fragment thereof and fragments of Sor h prepared synthetically. Sor h I and fragments thereof are useful for diagnosing, treating, and preventing allergy to Johnson grass pollen.
Claims13
An isolated nucleic acid comprising a nucleotide sequence coding for a Johnson grass pollen allergen Sor h I, wherein said nucleotide sequence is selected from the group consisting of the nucleotide sequence shown in FIG. 5 (SEQ ID NO:
- 1) and the coding region of the nucleotide sequence shown in FIG. 5 (SEQ ID NO:
- 1).
An isolated nucleic acid comprising a nucleotide sequence coding for a mature Johnson grass pollen allergen Sor h I, wherein the mature allergen comprises amino acid residues 1-238 of the amino acid sequence shown in FIG. 5 (SEQ ID NO:
- 2).
A method of producing a Johnson grass pollen allergen, Sor h I, comprising the steps of:
- a) culturing a host cell transformed with DNA comprising the nucleotide sequence shown in FIG. 5 (SEQ ID NO:
- 1), or the coding region thereof, in an appropriate medium to produce a mixture of cells and medium containing Sor h I protein;
- and
- b) purifying said mixture to produce substantially pure Sor h I protein.
A host cell transformed with a vector containing the cDNA insert of Sor h I clone 3S, said host cell having ATCC Deposit No. 69106 deposited Oct. 28, 1992.
An isolated nucleic acid comprising a nucleotide sequence coding for a Johnson grass pollen allergen Sor h I having the amino acid sequence shown in FIG. 5 (SEQ ID NO:
- 2).
An isolated nucleic acid comprising a nucleotide sequence coding for a naturally occurring polymorphic variant of a Johnson grass pollen allergen Sor h I, wherein the nucleotide sequence differs due to natural allelic variation from the nucleotide sequence shown in FIG. 5 (SEQ ID NO:
- 1).
An isolated nucleic acid comprising a nucleotide sequence coding for a protein allergen, said nucleotide sequence having sufficient homology to hybridize in 1.times.SSC, 0.1% SDS at 68.degree. C. for at least 15 minutes to the nucleotide sequence shown in FIG. 5 (SEQ ID NO:
- 1), with the proviso that the nucleic acid is not from Lolium perenne or Cynodon dactylon.
An isolated nucleic acid encoding a protein allergen comprising at least one T cell epitope present in a Johnson grass pollen allergen, Sor h I, wherein said Sor h I allergen comprises the amino acid sequence shown in FIG. 5 (SEQ ID NO:
- 2), with the proviso that the nucleic acid is not from Lolium perenne or Cynodon dactylon.
Dependencies are inferred from the claim text.