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US 5,691,158 AGranted
System and method for determining efficacy of sunscreen formulations
AssigneeMary Kay Cosmetics, Inc.
InventorsBarry T. Reece, Michael G. Rozen, David A. Deeds, Dale K. Roberts
Life of this patent
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Abstract
A system and method is disclosed employing a full thickness skin model for determining minimum sun protection factors (SPFs) of various sunscreen formulations by measuring the release of an inflammatory mediator in the skin model.
Claims33
A system for testing efficacy of a sunscreen formulation comprising:
- (a) means for generating ultraviolet radiation;
- (b) means, coupled to the means for generating ultraviolet radiation, for containing at least one artificial skin culture;
- (c) means, interposed between the means for generating ultraviolet radiation and the means for containing the at least one artificial skin culture, for holding a sunscreen formulation under test;
- (d) means for incubating the at least one artificial skin culture after exposure to the means for generating ultraviolet radiation;
- and
- (e) means for assaying the at least one artificial skin culture for an inflammatory mediator.
3 more elements
A method for testing efficacy of a sunscreen formulation comprising the steps of:
- (a) generating ultraviolet radiation for passing through the sunscreen formulation;
- (b) exposing at least one artificial skin culture to the ultraviolet radiation being passed through the sunscreen formulation under test, the sunscreen formulation being spaced from the artificial skin;
- (c) incubating the at least one artificial skin culture at predetermined conditions;
- (d) assaying the at least one artificial skin culture for an inflammatory mediator;
- and
- (e) correlating the inflammatory mediator assayed for to the efficacy of the sunscreen formulation.
3 more elements
An in vitro method for determining efficacy of a sunscreen formulation comprising the steps of:
- (a) placing at least one artificial skin culture into a culture tray;
- (b) affixing at least one strip of specimen tape to a glass plate;
- (c) applying a sunscreen formulation to at least one strip of specimen tape;
- (d) covering the culture tray with the glass plate;
- (e) exposing at least one artificial skin culture to ultraviolet radiation being passed through the sunscreen formulation;
- (f) incubating at least one artificial skin culture for a predetermined period of time, at a predetermined temperature, in a predetermined atmosphere;
- (g) assaying at least one artificial skin culture for an inflammatory mediator;
- and
- (h) correlating the inflammatory mediator assayed for to the efficacy of the sunscreen formulation.
6 more elements
An in vitro method of determining a sun protection factor for a sunscreen formulation comprising the steps of:
- exposing a plurality of artificial skin cultures through a sunscreen formulation under test, the sunscreen formulation being spaced from the artificial skin cultures, to ultraviolet radiation of substantially constant intensity, the plurality of artificial skin cultures being exposed for overlapping but separate increasingly spaced predetermined periods of time;
- assaying the plurality of artificial skin cultures for presence of an inflammatory mediator;
- identifying an artificial skin culture which releases the inflammatory mediator and its corresponding exposure time to ultraviolet radiation;
- and
- scaling the corresponding exposure time by a predetermined constant to obtain a sun protection factor.
2 more elements
A system for testing the efficacy of a sunscreen formulation comprising:
- (a) first means for generating ultraviolet radiation;
- (b) second means disposing at least one artificial skin culture in the path of said ultraviolet radiation;
- and
- (c) third means holding said sunscreen formulation under test interposed between said first ultraviolet radiation generating means and said second artificial skin culture disposing means, and being spaced from said artificial skin culture within said path.
1 more element
A method for testing efficacy of a sunscreen formulation comprising:
- (a) generating ultraviolet radiation for passing through said sunscreen formulation;
- (b) exposing, for a period of time, at least one artificial skin culture to said ultraviolet radiation being passed through said sunscreen formulation under test, the sunscreen formulation being disposed on a surface other than said artificial skin, said surface being disposed such that said ultraviolet radiation to which said artificial skin is exposed passes through said sunscreen formulation;
- (c) assaying said at least one artificial skin culture for an inflammatory mediator;
- and
- (d) correlating said inflammatory mediator assayed for to said period of time for which said at least one artificial skin culture was exposed to said ultraviolet radiation to determine the efficacy of said sunscreen formulation.
2 more elements
Dependencies are inferred from the claim text.