Method of inhibiting cell growth with the P.sub.2U receptor
AssigneesThe University of North Carolina at Chapel Hill, The Curators of the University of Missouri
InventorsRichard C. Boucher, Gary A. Weisman, John T. Turner, Thomas K. Harden, Claude E. Parr, Daniel M. Sullivan, Laura J. Erb, Kevin D. Lustig
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Abstract
Isolated DNAs encoding the human P.sub.2U receptor are disclosed, along with vectors and host cells containing the same and methods of using the same. Host cells which are essentially free of endogenous P.sub.2U receptor expression, and which express a heterologous P.sub.2U receptor such as a murine P.sub.2U receptor, are also disclosed, along with methods of using the same.
Claims1
A method of inhibiting the growth, of a cell in culture comprising:
- transforming a mammalian cell in culture with a vector that expresses a P.sub.2U receptor in said cell in an amount sufficient to inhibit cell growth by autostimulation of said P.sub.2U receptor,
- wherein said vector contains and expresses DNA encoding a human P.sub.2U receptor selected from the group consisting of;
- (a) isolated DNA having SEQ ID NO:1;
- and
- (b) isolated DNA differing from the isolated DNA of (a) above in nucleotide sequence due to the degeneracy of the genetic code;
- wherein said cell secretes ATP or UTP, and wherein cell growth is inhibited by stimulation of said P.sub.2U receptor with ATP or UTP.
Dependencies are inferred from the claim text.