Immunoassay for insulin-like growth factors
AssigneeDaiichi Radioisotope Laboratories, Ltd.
InventorsKazumine Mori, Shigeto Takasu, Hiroyuki Kasahara, Shuji Horikawa
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Abstract
Described are immunoassay of insulin-like growth factors in a biological sample, which comprises prior to the immunoassay, treating the biological sample with an acid solution to liberate the insulin-like growth factors from insulin-like growth factor binding proteins and then neutralizing the so-treated biological sample with a neutralizing buffer containing a recombination inhibitor; and immunoassay kit for insulin-like growth factors.
Claims17
An immunoassay for the insulin-like growth factors, IGF-I or IGF-II, or both in a biological sample, which comprises:
- (a) treating a biological sample with an acid solution to liberate IGF-I or IGF-II or both from binding proteins therefor;
- (b) neutralizing the acid treated biological sample with a neutralizing buffer containing a recombination inhibitor other than IGF-I or IGF-II;
- and
- (c) conducting an immunoassay for IGF-I or IGF-II or both;
- wherein purification of the liberated IGF-I or IGF-II of both from the binding proteins therefor is not required.
A process for the preparation of a biological sample for the detection of the insulin-like growth factors, IGF-I or IGF-II or both, which comprises:
- (a) treating the biological sample with an acid solution to liberate IGF-I or IGF-II or both from binding proteins therefor;
- and
- (b) neutralizing the acid treated biological sample in the presence of a recombination inhibitor other than IGF-I or IGF-II;
- and
- wherein purification of the liberated IGF-I or IGF-II or both from the binding proteins therefor is not required.
Dependencies are inferred from the claim text.