Petroleum immunoassay method, its components and a kit for use in performing the same
AssigneeEnsys, Inc.
InventorsStephen B. Friedman, Randy L. Allen
Life of this patent
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Abstract
An immunoassay method that integrates a sample processing component that enables the testing of a variety of environmental matrixes and the components for performing the method, wherein the immunoassay utilizes a monoclonal anti-petroleum antibody to detect the presence or absence of petroleum based contamination in a sample when tested in a field or laboratory location.
Claims16
A monoclonal antibody useful for detecting the presence of petroleum-based fuel in a sample, which specifically binds a characteristic plurality of compounds found in petroleum-based fuel, said characteristic plurality of compounds being a C.sub.1-2 alkyl substituted monocyclic aromatic hydrocarbon and unsubstituted bi- and tri-cyclic aromatic hydrocarbons, wherein said monoclonal antibody has a cross reactivity with benzene of <1%, wherein said monoclonal antibody is obtained by a process comprising:
- (i) producing an immune response in a vertebrate host by immunization with an immunogen comprised of a derivative moiety of the formula. ##STR6## wherein R.sup.1 to R.sup.5 are each independently H, CH.sub.3 or CH.sub.2 CH.sub.3 ;
- X and Y are each, independently ##STR7## or a single bond wherein R.sup.6 is selected from the group consisting of H, C.sub.1 -C.sub.2 alkyls, and linear, branched and cyclic C.sub.3 -C.sub.6 alkyls;
- and
- n is 0 or an integer from 1 to 4, linked to an immunogen carrier molecule;
- (ii) preparing a hybridoma from the lymphoid cells of said host;
- (iii) selecting said hybridoma to produce said monoclonal antibody;
- and
- (iv) obtaining said monoclonal antibody.
A monoclonal antibody which specifically binds toluene, xylene, ethylbenzene, naphthalene, methylnaphthalene, phenanthrene, and acenaphthene, wherein said monoclonal antibody has a cross reactivity with benzene of <1% , wherein said monoclonal antibody is obtained by a process comprising:
- (i) producing an immune response in a vertebrate host by immunization with an immunogen comprised of a derivative moiety of the formula ##STR8## wherein R.sup.1 to R.sup.5 are each independently H, CH.sub.3 or CH.sub.2 CH.sub.3 ;
- X and Y are each, independently ##STR9## or a single bond wherein R.sup.6 is selected from the group consisting of H, C.sub.1 -C.sub.2 alkyls, and linear, branched and cyclic C.sub.3 -C.sub.6 alkyls:
- and
- n is 0 or an integer from 1 to 4, linked to an immunogen carrier molecule;
- (ii) preparing a hybridoma from the lymphoid cells of said host;
- (iii) selecting said hybridoma to produce said monoclonal antibody;
- and
- (iv) obtaining said monoclonal antibody.
An enzyme immunoassay kit useful for detecting the presence of petroleum-based fuel in a sample, comprising a monoclonal antibody which specifically binds toluene, xylene, ethylbenzene, naphthalene, methylnaphthalene, phenanthrene, and acenaphthene, wherein said monoclonal antibody is obtained by a process comprising:
- (i) producing an immune response in a vertebrate host by immunization with an immunogen comprised of a derivative moiety of the formula ##STR10## wherein R.sup.1 to R.sup.5 are each independently H, CH.sub.3 or CH.sub.2 CH.sub.3 ;
- X and Y are each, independently ##STR11## or a single bond wherein R.sup.6 is selected from the group consisting of H, C.sub.1 -C.sub.2 alkyls, and linear, branched and cyclic C.sub.3 -C.sub.6 alkyls;
- and
- n is 0 or an integer from 1 to 4, linked to an immunogen carrier molecule;
- (ii) preparing a hybridoma from the lymphoid cells of said host;
- (iii) selecting said hybridoma to produce said monoclonal antibody;
- and
- (iv) obtaining said monoclonal antibody.
A monoclonal antibody useful for detecting the presence of petroleum-based fuel in a sample, which specifically binds a characteristic plurality of compounds found in petroleum-based fuel, said characteristic plurality of compounds being a C.sub.1-2 alkyl substituted monocyclic aromatic hydrocarbon, unsubstituted bi- and tri-cyclic aromatic hydrocarbons and a C.sub.6-8 aliphatic hydrocarbon selected from the group consisting of n-heptane, 2-methylhexane, 2-methylpentane and n-octane, wherein said monoclonal antibody specifically binds said characteristic plurality of compound to provide substantially equivalent detection of petroleum-based fuels, said petroleum based fuels being gasoline, diesel, kerosene, jet fuel and fuel oil #2 wherein said monoclonal antibody has a cross reactivity with benzene of <1% .
Dependencies are inferred from the claim text.