Poliovirus specific primers and methods of detection utilizing the same

A method for detecting the presence or absence of poliovirus serotype 1 in a sample containing nucleic acids comprising the steps of:

• a) amplifying the nucleic acids from the sample with a primer pair comprised of a first primer comprised of the nucleotide sequence set forth in the Sequence Listing as SEQ ID NO:
• 23 and a second primer comprised of the nucleotide sequence set forth in the Sequence Listing as SEQ ID NO:
• 22;
• and
• b) determining the presence or absence of nucleic acid from poliovirus serotype 1, thereby detecting the presence or absence of poliovirus serotype 1 in the sample.

A method for detecting the presence or absence of poliovirus serotype 2 in a sample containing nucleic acids comprising the steps of:

• a) amplifying the nucleic acids from the sample with a primer pair comprised of a first primer comprised of the nucleotide sequence set forth in the Sequence Listing as SEQ ID NO:
• 25 and a second primer comprised of the nucleotide sequence set forth in the Sequence Listing as SEQ ID NO:
• 24;
• and
• b) determining the presence or absence of nucleic acid from poliovirus serotype 2, thereby detecting the presence or absence of poliovirus serotype 2 in the sample.

A method for detecting the presence or absence of poliovirus serotype 3 in a sample containing nucleic acids comprising the steps of:

• a) amplifying the nucleic acids from the sample with a primer pair comprised of a first primer selected from the group consisting of a primer comprised of the nucleotide sequence set forth in the Sequence Listing as SEQ ID NO:
• 27 and a primer comprised of the nucleotide sequence set forth in the Sequence Listing as SEQ ID NO:
• 28, and a second primer comprised of the nucleotide sequence set forth in the Sequence Listing as SEQ ID NO:
• 26;
• and
• b) determining the presence or absence of nucleic acid from poliovirus serotype 3, thereby detecting the presence or absence of poliovirus serotype 3 in the sample.