Low pathogencity PRRS live virus vaccines and methods of preparation thereof
AssigneeRegents of the University of Minnesota
InventorHan Soo Joo
Life of this patent
Estimating the term…
Abstract
Live or modified live PRRS vaccines for administration to swine are provided which are of low virulence and confer effective immunity against PRRS. The preferred vaccines include virus isolates having average plaque diameters of less than about 2 mm and low pathogenicity. A preferred vaccine includes a small plaque diameter strain, ATCC Accession No. VR2509. The vaccines of the invention may be administered to breeding females or gilts and to weaned piglets, and is effective to immunize the swine against both the respiratory and reproductive forms of the disease.
Claims12
A vaccine comprising a virus having the designation ATCC VR2509.
A vaccine comprising a North American porcine reproductive and respiratory syndrome virus (PRRSV) strain, wherein the strain gives plaques having an average diameter of less than 2 mm when the plaques are obtained by a method comprising the steps of:
- (a) inoculating an aliquot of cells with the strain, wherein the cells have the designation ATCC CRL-12219, wherein the aliquot is obtained from a confluent monolayer of the cells maintained in Eagle's minimum essential medium supplemented with 3% fetal calf serum and 0.15% sodium carbonate, wherein the aliquot includes 1-2 X 10.sup.5 cells per ml of Eagle's minimum essential medium supplemented with 3% fetal calf serum and 0.15% sodium carbonate;
- (b) incubating the aliquot at 37.degree. C. for 60 minutes;
- (c) removing the inoculum from the aliquot;
- (d) washing the aliquot with Eagle's minimum essential medium;
- (e) resuspending the aliquot in 5 ml of 2X Eagle's minimum essential medium and 1.6% boiled Noble agar supplemented with 250 .mu.g of diethylaminoethyl (DEAE)-dextran;
- (f) plating the aliquot;
- and
- (g) further incubating the aliquot at 37.degree. C. for 5 days in a CO.sub.2 incubator to give the plaques.
An isolated and purified North American porcine reproductive and respiratory Syndrome virus (PRRSV) strain, wherein the strain has the designation ATCC VR2509.
An isolated and purified North American porcine reproductive and respiratory syndrome virus (PRRSV) strain, wherein the strain gives plaques having an average diameter of less than 2 mm when the plaques are obtained by a method comprising the steps of:
- (a) inoculating an aliquot of cells with the strain, wherein the cells have the designation ATCC CRL-12219, wherein the aliquot is obtained from a confluent monolayer of the cells maintained in Eagle's minimum essential medium supplemented with 3% fetal calf serum and 0.15% sodium carbonate, wherein the aliquot includes 1-2 X 10.sup.5 cells per ml of Eagle's minimum essential medium supplemented with 3% fetal calf serum and 0.15% sodium carbonate;
- (b) incubating the aliquot at 37.degree. C. for 60 minutes;
- (c) removing the inoculum from the aliquot;
- (d) washing the aliquot with Eagle's minimum essential medium;
- (e) resuspending the aliquot in 5 ml of 2X Eagle's minimum essential medium and 1.6% boiled Noble agar supplemented with 250 .mu.g of diethylaminoethyl (DEAE)-dextran;
- (f) plating the aliquot;
- and
- (g) further incubating the aliquot at 37.degree. C. for 5 days in a CO.sub.2 incubator to give the plaques.
Dependencies are inferred from the claim text.