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US 5,690,926 AGranted
Pluripotential embryonic cells and methods of making same
AssigneeVanderbilt University
InventorBrigid L. M. Hogan
Life of this patent
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Abstract
The claimed invention is directed towards non-murine pluripotential cells that have the ability to be passaged in vitro for at least 20 passages and which differentiate in culture into a variety of tissues. The scope of the claimed cells includes any non-murine ES cells and particular claims are drawn to human pluripotential cells.
Claims7
An isolated non-murine mammalian pluripotential cell wherein said cell exhibits the following characteristics:
- (a) can be maintained on feeder layers for at least 20 passages;
- and
- (b) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture;
- and wherein said cell is derived from a primordial germ cell by the process of:
- (1) culturing a non-murine mammalian primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor;
- (2) selecting cells that have characteristics (a) and (b), above, and
- (3) isolating said non-murine pluripotential cell.
4 more elements
An isolated human pluripotential cell wherein said cell exhibits the following characteristics:
- (a) can be maintained on feeder layers for at least 20 passages;
- and
- (b) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture;
- and wherein said cell is derived from a human primordial germ cell by the process of:
- (1) culturing a human primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor;
- (2) selecting cells that have characteristics (a) and (b), above, and
- (3) isolating said human pluripotential cell.
4 more elements
A composition comprising:
- (A) a human pluripotential cell derived from a primordial germ cell wherein said cell exhibits the following characteristics:
- (1) can be maintained on feeder layers for at least 20 passages;
- and
- (2) gives rise to embryoid bodies and differentiated cells of multiple phenotypes in monolayer culture;
- and wherein said cell is derived from a primordial germ cell by the process of:
- (a) culturing a human primordial germ cell in a composition comprising basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor;
- (b) selecting cells that have characteristics (a) and (b), above, and
- (c) isolating said human pluripotential cell;
- and
- (B) a fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor, each in amounts sufficient to permit continued proliferation of said cell.
7 more elements
Dependencies are inferred from the claim text.