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AssigneesHer Majesty the Queen in right of Canada, as represented by Agriculture and Agri-Food, National Research Council of Canada
InventorsLaurian S. Robert, Jean L. Gerster, Hai Ping Hong
Estimating the term…
A Brassica sp. genomic clone containing a polygalacturonase gene promoter was isolated. This promoter directs high levels of transcription in the pollen. When the promoter was fused with the GUS gene and introduced into B. napus by Agrobacterium mediated transformation, this promoter controlled GUS expression in microspores and pollen in transgenic B. napus plants. Expression occurred between the late uninucleate microspore stage and the mature pollen grain stage. GUS activity was also identified in tapetal tissue at the binucleate microspore stage. There was no GUS expression in other tissues such as root, stem, leaf, sepal, petal or pistil. This promoter will be useful for the temporal and spatial control of endogenous gene expression in plants.
An isolated and purified Brassica sp. polygalacturonase promoter, which directs transcription in pollen wherein said promoter has a nucleotide sequence which is at least 80% homologous to SEQ ID NO:
A chimeric gene construct comprising a Brassica sp. polygalacturonase gene promoter and a coding sequence of an exogenous gene, wherein said promoter directs transcription of the exogenous gene in the pollen and has a nucleotide sequence which is at least 80% homologous to SEQ ID NO:
A method of conferring pollen-directed expression of a gene in a plant, comprising:
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