Production of gamma linolenic acid by a .DELTA.6-desaturase
AssigneeRhone-Poulenc Agrochimie
InventorsTerry L. Thomas, Avutu S. Reddy, Michael Nuccio, Georges L. Freyssinet
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Abstract
Linoleic acid is converted into .gamma.-linolenic acid by the enzyme .DELTA.6-desaturase. The present invention is directed to an isolated nucleic acid comprising the .DELTA.6-desaturase gene. More particularly, the isolated nucleic acid comprises the promoter, coding region and termination regions of the .DELTA.6-desaturase gene. The present invention provides recombinant constructions comprising the .DELTA.6-desaturase coding region in functional combination with heterologous regulatory sequences. The nucleic acids and recombinant constructions of the instant invention are useful in the production of GLA in transgenic organisms.
Claims18
A transgenic plant or bacteria comprising at least one of a DNA molecule encoding cyanobacterial .DELTA.6-desaturase wherein said DNA molecule is isolated from a cyanobacterium that produces gamma linolenic acid;
- a DNA molecule having the sequence of SEQ ID NO:3;
- or a DNA molecule encoding a Synechocystis .DELTA.6-desaturase.
A transgenic plant or bacteria comprising at least one of a vector comprising a DNA molecule encoding cyanobacterial .DELTA.6-desaturase wherein said DNA molecule is isolated from a cyanobacterium that produces gamma linolenic acid;
- a vector comprising a DNA molecule having the sequence of SEQ ID NO:3;
- or a vector comprising a DNA molecule encoding a Synechocystis .DELTA.6-desaturase.
A plant or part thereof which has been regenerated from a plant cell stably transformed with a vector selected from the group consisting of a vector comprising a DNA molecule encoding cyanobacterial .DELTA.6-desaturase wherein said DNA molecule is isolated from a cyanobacterium that produces gamma linolenic acid;
- a vector comprising a DNA molecule having the sequence of SEQ ID NO:3;
- or a vector comprising a DNA molecule encoding a Synechocystis .DELTA.6-desaturase.
A method of producing a plant with increased gamma linolenic acid (GLA) content which comprises:
- transforming a plant cell with at least one of an isolated DNA molecule having the sequence of SEQ ID NO:3 or an isolated DNA molecule encoding a Synechocystis .DELTA.6-desaturase
- and regenerating a plant with increased GLA content from said plant cell.
A method of producing a plant with increased gamma linolenic acid (GLA) content which comprises transforming a plant cell with at least one of a vector comprising an isolated DNA molecule having the sequence of SEQ ID NO:3 or a vector comprising an isolated DNA molecule encoding a Synechocystis .DELTA.6-desaturase;
- and regenerating a plant with increased GLA content from said plant cell.
A method of inducing production of gamma linolenic acid (GLA) in a plant deficient or lacking in GLA which comprises transforming said plant with at least one of an isolated DNA molecule having the sequence of SEQ ID NO:3or an isolated DNA molecule encoding a Synechocystis .DELTA.6-desaturase.
A method of inducing production of gamma linolenic acid (GLA) in a plant deficient or lacking in GLA which comprises transforming said plant with at least one of a vector comprising an isolated DNA molecule having the sequence of SEQ ID NO:3 or a vector comprising an isolated DNA molecule encoding a Synechoystis .DELTA.6-desaturase.
A method of inducing production of gamma linolenic acid(GLA) in a plant deficient or lacking in GLA and linoleic acid (LA) which comprises transforming said plant with an isolated DNA molecule encoding a Synechocystis .DELTA.6-desaturase and an isolated DNA molecule encoding .DELTA.12-desaturase.
A method of inducing production of gamma linolenic acid (GLA) in a plant deficient or lacking in GLA and linoleic acid (LA) which comprises transforming said plant with at least one expression vector comprising an isolated DNA molecule encoding a Synechocystis .DELTA.6-desaturase and an isolated DNA molecule encoding .DELTA.12-desaturase.
A method of inducing production of octadecatetraeonic acid in a plant deficient or lacking in gamma linolenic acid which comprises transforming said plant with at least one of an isolated DNA molecule having the sequence of SEQ ID NO:3 or an isolated DNA molecule encoding a Synechocystis .DELTA.6-desaturase.
A method of inducing production of octadecatetraeonic acid in a plant deficient or lacking in gamma linolenic acid which comprises transforming said plant with at least one of a vector comprising an isolated DNA molecule having the sequence of SEQ ID NO:3 or a vector comprising an isolated DNA molecule encoding a Synechosystis .DELTA.6-desaturase.
Dependencies are inferred from the claim text.