Transgenic pathogen-resistant plant
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften
InventorsJurgen Logemann, Guido Jach, Birgit Gornhardt, John Mundy, Jeff Schell, Peter Eckes, Ilan Chet
Life of this patent
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Abstract
Transgenic pathogen-resistant organism whose genome contains at least two different genes under the control of active promoters with pathogen-inhibiting action. This organism is distinguished by a synergistic pathogen-inhibiting action. This action is evident particularly when the genes code for the gene products chitinase (ChiS, ChiG), glucanase (GluG), protein synthesis inhibitor (PSI) and antifungal protein (AFP).
Claims9
A transgenic plant comprising
- (a) at least one gene, operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and
- (b) a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, operably linked to a plant-functional promoter, wherein the plant is resistant to fungal attack.
A transgenic plant comprising
- (a) at least one gene operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, and
- (b) a AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, operably linked to a plant-functional promoter, wherein the plant is resistant to fungal attack.
A purified and isolated DNA molecule comprising
- (a) at least one gene selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and
- (b) a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8.
A purified and isolated DNA molecule comprising
- (a) at least one gene selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, and
- (b) an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1.
A process for the generation of a transgenic fungus-resistant plant comprising employing a DNA-vector comprising
- (a) at least one gene operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4 and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, and
- (b) an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, operably linked to a plant-functional promoter, for transformation of a plant.
A process for the generation of transgenic fungus-resistant plant, comprising employing a DNA vector comprising
- (a) at least one gene operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4 and an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and
- (b) a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, operably linked to a plant-functional promoter, for transformation of a plant.
Dependencies are inferred from the claim text.