Linker for immobilization, modification and subsequent release of oligomers with a terminal hydroxyl group

A method for the immobilization and subsequent release of an organic hydroxylic compound, R--OH, comprising the following steps:

• i) converting the molecule R--OH into its phosphoramidite derivative R--O--P(OR')--N(R").sub.2 wherein R is a primary, secondary, or tertiary aliphatic or aromatic group which may or may not contain other functional groups;
• wherein R' is selected from the group consisting of methyl, ethyl, cyanoethyl, phenyl, chlorophenyl, and other aliphatic, branched aliphatic or aromatic groups;
• wherein R" is selected from the group consisting of methyl, ethyl, isopropyl and other linear and branched aliphatic groups;
• ii) a hydroxyl protecting group Y is selectively removed from a derivatized solid support having the structure ##STR4## where a+b is greater than 1 but less than 6, and X is a nitrogen or CH group, wherein said structure is attached to a solid support --R by a bridge group selected from the group consisting of bifunctional aliphatic, bifunctional branched aliphatic, bifunctional polyethylene glycol containing and bifunctional aromatic groups;
• iii) said phosphoramidite is attached to said hydroxyl deprotected derivatized solid support by activation with a tetrazole solution or other phosphoramidite activator solution, followed by oxidation of the phosphorus with iodine solution or other oxidant solution to produce a compound having the structure ##STR5## iv) the protecting group Z of the intermediate produced by step iii which masks the nitrogen functionality is removed selectively, and;
• v) the intermediate produced by step iv is contacted with a mildly basic buffer solution to promote intramolecular attack at phosphorus by the nitrogen to produce the compound R--OH.