Insecticidal peptides from spider venom
AssigneeNPS Pharmaceuticals, Inc.
InventorsJanice H. Johnson, Robert M. Kral, Jr., Karen Krapcho
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Abstract
This invention relates to the purification of a family of insecticidally effective peptides isolated from the spider, Calisoga sp., characterized by their neurotoxic effect on insect pest and low mammalian toxicity. The cDNA sequences for three of these peptides have been isolated, and the complete coding sequence is provided. This invention also discloses methods for producing recombinant peptides, as well as methods of utilizing these peptides as insecticidal agents.
Claims38
A fraction of whole Calisoga spider venom comprising a fraction which is neurotoxic to Heliothis virescens, said neurotoxic fraction comprising SEQ NO:1, SEQ ID NO:2 or SEQ ID NO:3.
A substantially purified insecticidally effective peptide isolated from Calisoga spider venom which is neurotoxic to insect pests, has an observed molecular mass of about 4300 amu and a PD.sub.50 in Heliothis virescens greater than about 2.37 .mu.g/g.
An insect toxin comprising the amino acid sequence encoded by SEQ ID NO:5 or fragment thereof which is toxic to insects.
An insect toxin comprising the amino acid sequence of SEQ ID NO:6 or a functional derivative or fragment thereof which is toxic to insects.
An insect toxin comprising the amino acid sequence encoded by SEQ ID NO:7 or fragment thereof which is toxic to insects.
A substantially purified insecticidally effective peptide isolated from Calisoga spider venom which is neurotoxic to insect pests, has an observed molecular mass of about 4300 amu and a PD.sub.50 in Heliothis virescens of about 3.7 .mu.g/g.
A substantially purified insecticidally effective peptide isolated from Calisoga spider venom which is neurotoxic to insect pests, has an observed molecular mass of about 4300 amu and a PD.sub.50 in Heliothis virescens of about 4.5 .mu.g/g.
A substantially purified insecticidally effective peptide isolated from Calisoga spider venom which is neurotoxic to insect pests, has an observed molecular mass of about 4300 amu and a PD.sub.50 in Heliothis virescens of about 2.37 .mu.g/g.
Dependencies are inferred from the claim text.